6 research outputs found
Relative mRNA expression quantification.
<p><i>RANKL</i> (A), <i>OPG</i> (B), <i>OC</i> (C), <i>COL1A</i> (D), <i>MMP-2</i> (E), and <i>MMP-9</i> (F) mRNA expression in bone tissue of control, type 1 diabetes mellitus (T1DM), and T1DM plus zinc supplementation (T1DMS) rats. All data are expressed as fold-change vs. control group values, normalized to <i>GAPDH</i>. Comparisons between groups were analyzed with Kruskal-Wallis ANOVA on Ranks and Dunn’s post-hoc. <i>p</i> < 0.05*<sup>/#</sup> vs. control group; <i>p</i> < 0.01*<sup>/##</sup> vs. control group.</p
Assessment of collagen deposition by picrosirius red staining.
<p>Tibia staining for collagen content (picrosirius red). Total collagen (A), collagen type I (B), and collagen type III (C) contents of the control, type 1 diabetes mellitus (T1DM), and T1DM plus zinc supplementation (T1DMS) groups. All data are shown as means ± SEM. Comparisons between groups were analyzed with Kruskal-Wallis ANOVA on Ranks and Dunn’s post-hoc. <i>p</i> < 0.05 *<sup>/#</sup> vs. control group.</p
Histological analyses of the right tibias.
<p>Hematoxylin and eosin staining of longitudinal sections of tibias of the control (A), type 1 diabetes mellitus (T1DM) (B), and T1DM plus zinc supplementation (T1DMS) (C) groups. TB, trabecular bone; MS, medullary space; magnification 20X, scale bar: 50 μm.</p
Histomorphometric analyses of structural bone architecture.
<p>Trabecular separation (TbSP, μm) (A), trabecular width (TbWi, μm) (B), and trabecular bone area (BAr, %) (C) of control, type 1 diabetes mellitus (T1DM), and T1DM plus zinc supplementation (T1DMS) rats. All data are shown as means ± SEM. Comparisons between groups were analyzed with Kruskal-Wallis ANOVA on Ranks and Dunn’s post-hoc. <i>p</i> < 0.01*<sup>/##</sup> vs. control group; <i>p</i> < 0.001*<sup>/###</sup> vs. control group; <i>p</i> < 0.05 **<sup>/#</sup> vs. T1DM group; <i>p</i> < 0.001**<sup>/###</sup> vs. T1DM group.</p
Tibia biomechanical parameters of control, diabetic, and diabetic plus zinc supplementation groups.
<p>T1DM, type 1 diabetes mellitus; T1DMS, T1DM plus zinc supplementation. All data are shown as means ± SEM. Comparisons between groups were analyzed with Kruskal-Wallis ANOVA on Ranks and Dunn’s post-hoc.</p><p>*<sup>/#</sup><i>p</i> < 0.05 vs. control group;</p><p>*<sup>/##</sup><i>p</i> < 0.01 vs. control group.</p><p>Tibia biomechanical parameters of control, diabetic, and diabetic plus zinc supplementation groups.</p
Biochemical analyses and body weight of control, diabetic, and diabetic plus zinc supplementation groups.
<p>T1DM, type 1 diabetes mellitus; T1DMS, T1DM plus zinc supplementation; ALP, alkaline phosphatase. All data are shown as means ± SEM. Comparisons between groups were analyzed with Kruskal-Wallis ANOVA on Ranks and Dunn’s post-hoc.</p><p>*<sup>/#</sup><i>p</i> < 0.05 vs. control group;</p><p>*<sup>/##</sup><i>p</i> < 0.01 vs. control group;</p><p>*<sup>/###</sup><i>p</i> < 0.001 vs. control group;</p><p>**<sup>/##</sup><i>p</i> < 0.01 vs. T1DM group.</p><p>Biochemical analyses and body weight of control, diabetic, and diabetic plus zinc supplementation groups.</p