10 research outputs found
2,4-Dichloro-N-phenethylÂbenzeneÂsulfonamide
In the title compound, C14H13Cl2NO2S, the dihedral angle between the phenyl ring and the benzene ring is 69.94â
(9)°. Two short intraÂmolecular CâHâŻO contacts occur and a weak interÂmolecular CâHâŻÏ interÂaction is seen in the crystal
Cytotoxicity and potential antiviral evaluation of violacein produced by Chromobacterium violaceum Â
Natural products are an inexhaustible source of compounds with
promising pharmacological activities including antiviral action.
Violacein, the major pigment produced by Chromobacterium violaceum, has
been shown to have antibiotic, antitumoral and anti-Trypanosoma cruzi
 activities. The goal of the present work was to evaluate the
cytotoxicity of violacein and also its potential antiviral
properties.The cytotoxicity of violacein was investigated by three
methods: cell morphology evaluation by inverted light microscopy and
cell viability tests using the Trypan blue dye exclusion method and the
MTT assay. The cytotoxic concentration values which cause destruction
in 50% of the monolayer cells (CC50) were different depending on the
sensitivity of the method. CC50 values were ℠2.07 ± 0.08
ΌM for FRhK-4 cells: ℠2.23 ± 0.11 ΌM for Vero
cells; â„ 2.54 ± 0.18 ÎŒM for MA104 cells; and â„
2.70 ± 0.20 ΌM for HEp-2 cells. Violacein showed no
cytopathic inhibition of the following viruses: herpes simplex virus
type 1 (HSV-1) strain 29-R/acyclovir resistant, hepatitis A virus
(strains HM175 and HAF-203) and adenovirus type 5 nor did it show any
antiviral activity in the MTT assay. However violacein did show a weak
inhibition of viral replication: 1.42 ± 0.68%, 14.48 ± 5.06%
and 21.47 ± 3.74% for HSV-1 (strain KOS); 5.96 ± 2.51%, 8.75
± 3.08% and 17.75 ± 5.19% for HSV-1 (strain ATCC/VR-733);
5.13 ± 2.38 %, 8.18 ± 1.11% and 8.51 ± 1.94% for
poliovirus type 2; 8.30 ± 4.24%; 13.33 ± 4.66% and 24.27
± 2.18% for simian rotavirus SA11, at 0.312, 0.625 and 1.250 mM,
respectively, when measured by the MTT assay