Co-localization of Tex1 with Rex1.

<p>P27-specific polyclonal mouse sera (in red) was used to detect TEX1. Rex1 polyclonal rabbit sera (in green). (A) Ring stage parasites; (B) trophozoite stages; (C) schizont stages. Scatter plots show the degree of co-localization of the Tex1 with Rex1 signal. Nuclear DNA was stained with DAPI (blue), Transmission image (DIC), Scale bar: 5 µm.</p

Time points of harvest of synchronized 3D7 <i>in vitro</i> culture.

<p>Synchronized <i>P. falciparum</i> 3D7 parasite culture were harvested in 4 hours interval. Time points of harvest 1–14 (column 1), the corresponding age of synchronized parasites at each time point of harvest (in hours post invasion, column 2) and the corresponding parasite stage (column 3).</p

Co-localization of Tex1 with SBP1.

<p>P27-specific polyclonal rabbit sera was used to detect Tex1 (red). Co-localization was performed using SBP1 polyclonal mouse sera (green). Co-localization was performed in ring (A) trophozoite (B) and schizont stage (C) infected RBCs. Nuclear DNA was stained with DAPI (blue), Transmission image (DIC), Scale bar: 5 µm.</p

Tex1 localization in trophozoite and schizont stages with respect to newly described structures called tethers.

<p>Co-localization of Tex1 (red) with MAHRP2 (green) A) in trophozoite stages and B) in schizont stages. Nuclear DNA was stained with DAPI (blue), Transmission image (DIC), Scale bar: 5 µm.</p

Cell immune response and association with HLA II epitopes prediction.

a<p>SFC: spot-forming colonies×10⁶; ^bNo response observed.</p

Immunogenicity of coiled coil peptides in BALB/c mice.

<p>Titration of IgG antibody responses to coiled coil peptides in immunized mice. Evaluation on days 0, 30 and 50. Titers shown are according to a Log₁₀ scale. “φ, η, β and τ corresponds to an identification mark for each one of the animals per group. ELISA experiments were performed by duplicated in two independent experiments.</p

Immunogenicity of <i>P. vivax</i> coiled coil fragments in BALB/c mice.

a<p>ID from PlasmoDB; ^b(-) negative, (+) positive with 1-10, and (++) positive between 10 to 20 fluorescent parasites per well, respectively.</p

Co-localization of Tex1 with MAHRP1.

<p>P27-specific polyclonal rabbit sera was used to detect Tex1 (red). Co-localization was performed using MAHRP1 polyclonal mouse sera (green). Co-localization was performed in ring stage (A) trophozoite (B) and schizont stage (C) infected RBC. Nuclear DNA was stained with DAPI (blue), Transmission image (DIC), Scale bar: 5 µm.</p

Schematic representation of the antigen selection process.

<p>Download selection is represented: first, 50 <i>P. vivax</i> antigens containing α-helical coiled coil motifs, selected from orthologues of <i>P. falciparum</i>, were chemically synthesized and tested with PNG sera. Seventeen reactive with prevalence >30% were tested with Colombian sera. Eight antigens with prevalence >50% either with PNG or Colombian sera were used for mice immunization and immune response testing. Four antigens were finally selected for further pre-clinical testing.</p

Representative CD spectra of peptides (A) <i>Pv</i>Pep40 and (B) <i>Pv</i>Pep63.

<p>The CD's were done at room temperature on 150 µg/mL samples in aqueous solutions. Spectrums came from the averages of duplicates in the far UVs, from 190 to 250 nm and were smoothed with a 5 points filter.</p