A diagram showing the set-up of the test-tank inside the larger water-tank with an aquarium heater and two circulation pumps placed to ensure a stable temperature.

<p>The diagram shows the side of the test-tank facing the camera.</p

Mean latency from being placed in the anaesthetic bath to loss of equilibrium, from being placed in the anaesthetic bath to loss of response to touch, and from return to the test tank to regaining equilibrium in tricaine -treated fish.

<p>Mean latency from being placed in the anaesthetic bath to loss of equilibrium, from being placed in the anaesthetic bath to loss of response to touch, and from return to the test tank to regaining equilibrium in tricaine -treated fish.</p

An overview of the experimental design.

<p>An overview of the experimental design.</p

Mean ± sd or median with the 25% quartile (Q1) and 75% quartile (Q3) for each of the behavioural variables.

<p>Mean ± sd or median with the 25% quartile (Q1) and 75% quartile (Q3) for each of the behavioural variables.</p

Ethogram of alert behaviours including definitions of proximity to novel object [20].

<p>Ethogram of alert behaviours including definitions of proximity to novel object <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107357#pone.0107357-Nicol2" target="_blank">[20]</a>.</p

Average egg weight and total egg production on a given day for aviary and cage reared birds at 20, 24, 28, 41 and 73 weeks of age.

<p>Average egg weight and total egg production on a given day for aviary and cage reared birds at 20, 24, 28, 41 and 73 weeks of age.</p

Ethogram of comfort and aversion-related behaviour [20].

<p>Ethogram of comfort and aversion-related behaviour <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107357#pone.0107357-Nicol2" target="_blank">[20]</a>.</p

Weight and food competition capacity in offspring

<p>Birds were weighed within an hour after hatching and at eight days of age. Food competition capacity was estimated as the percentage of time in which each individual occupied the feeder in a pair-wise competition test. The data were analysed with ANOVA, using breed and parental treatment as fixed independent variables. Data (LS Means±SEM) with different superscripts in the table differ significantly at p<0.05.</p

Figure 2

<p>Correlations between magnitude of differential expression of genes between parents and offspring. Diagrams show M-values for the differential expression (comparing stressed vs control parents, and offspring of stressed vs offspring of control parents) of the 500 most differentially expressed genes (largest log₂ difference caused by stress in parents) in (a) red junglefowl, and (b) White Leghorns. Each point represents one spot on the microarray. Positive M-values indicate upregulation and negative downregulation by stress (or by having stressed parents). The average correlation line is shown in both comparisons.</p

Genes differentially expressed both in White Leghorn parents and offspring.

<p>The table shows the 10 genes with highest differential expression in WL offspring, out of those which were among the 100 most differentially expressed genes in both WL parents and offspring. Negative M-values indicate that the expression level was higher in control than in stress birds.</p