PLA Figure 2 Negative Series

The second set of images provided is a set of photomicrographs acquired on methanol fixed T47D breast cancer cells and subjected to proximity ligation assays using antibodies to detect interactions between CDK2 and Cyclin E1 and counter stained with ToPro3/DAPI (nuclei) and Phalloidin (cytoplasm)

IHC Figure 1 Model 1 Control Photomicrographs

The first set of photomicrographs provided depict the lungs from mice bearing human breast cancer cell line xenografts subjected to DAB+ immune-histochemistry using antibodies to either anti-human high molecular weight cytokeratin (MDA-MB-468 cells, model 1) or anti-human Vimentin (MDA-MB-231 cells, model 2), a technique that was used to quantify metastatic deposits in the lungs

IHC Figure 1 Model 1 Treated Photomicrographs

The first set of photomicrographs provided depict the lungs from mice bearing human breast cancer cell line xenografts subjected to DAB+ immune-histochemistry using antibodies to either anti-human high molecular weight cytokeratin (MDA-MB-468 cells, model 1) or anti-human Vimentin (MDA-MB-231 cells, model 2), a technique that was used to quantify metastatic deposits in the lungs

PLA Figure 2 Positive Series

The second set of images provided is a set of photomicrographs acquired on methanol fixed T47D breast cancer cells and subjected to proximity ligation assays using antibodies to detect interactions between CDK2 and Cyclin E1 and counter stained with ToPro3/DAPI (nuclei) and Phalloidin (cytoplasm

IHC Figure 1 Model 2 Treated Photomicrographs

The first set of photomicrographs provided depict the lungs from mice bearing human breast cancer cell line xenografts subjected to DAB+ immune-histochemistry using antibodies to either anti-human high molecular weight cytokeratin (MDA-MB-468 cells, model 1) or anti-human Vimentin (MDA-MB-231 cells, model 2), a technique that was used to quantify metastatic deposits in the lungs