Role of Systemic Vasoconstruction in Regulatory Installation of Blood Circulation

The aim of our study was an anthropophysiological justification for the hemodynamic basis of the pressor (hyperresistive) installation in the circulatory state of the cardio-vascular system (CVS). Materials and methods. For the analysis, prefabricated materials were used according to agespecific blood pressure standards (BP), supplemented with their calculated data on the yearly increase in blood pressure values and were combined with the stages of postnatal adaptation of the CVS for the gravitational factor of the blood circulation. Based on the clinical observational study, an anthropophysiological diagnosis of the circulatory state of the CVS with a systemic assessment of the arterial impedance were carried out for the main circulatory blocks of the blood circulation (head, lungs, abdomen, pelvis, thigh, shin). Results. It was shown that with a permanent increase in blood pressure during postnatal ontogenesis, its highest increments were noted at the initial stages of the formation of the upright. This dynamic was based on a system pressor set in the regulation of the circulatory state of the CVS, which manifested itself, especially when standing, in the prevalence of hyperresistive syndromes of arterial vessels. The most pronounced throughout postnatal ontogenesis, hyperresistant syndromes in the standing position were manifested in the pelvis and lower limbs regions. Conclusions. Anthropophysiological approach, including syndromic analysis, were allowed early diagnosis of circulatory conditions that underlie circulatory failure

Specialties of Blood Serum Structurization Under Some Physiological Conditions

Background: it is well-known that physiological stress agents can to produce a significant response of regulatory systems. At the same time, the nature of metabolic shifts in the organism during significant physical exertion and emotional stress has been insufficiently studied in detail. Aim: The aim of this investigation is study of modification of crystallogenic properties of human blood serum action under physical exertion and psychoemotional stress. Results: it is stated that physical exertion and psychoemotional stress causes increasing of crystallogenic activity of human blood serum. This trend is more pronounced for the first impact. This is confirmed by the results of vizuametric analysis of blood serum facias and its spectrometric testing. Conclusion: we show that crystalloscopic picture of blood serum is an informative criterion of different physiological conditions, including physical exertion and psychoemotional stress. It realized in the shifts of structure of biological fluid facias

The Study of Dielectric Properties of Biological Tissue under Thermal Modification in vitro

The aim of this paper is to study the dynamics of the dielectric properties of the tissue in the experimental controlled thermal modification. Material and methods: the experiment was performed on equal in volume and mass samples of intraoperatively removed tissue of Palmar aponeurosis (n=8). The thermal effect was simulated by placing tissue fragments in a thermostat (processing time – 5 min., temperature – 60 C). Duration of exposure after thermal modification was 5 min. Dielectric properties of tissues was determined using an original hardware-software complex for near-field resonant microwave sensing. Results: the study made it possible to verify the shifts in dielectric properties of the tissue that occur under short-term exposure to high temperature in vitro. It is shown that the dielectric permeability and conductivity of the biological object are significantly reduced under the influence of this factor, which is primarily due to a decrease of its hydration degree

Crystalloscopic analysis in detection of saliva metabolome specialties in elderly people

The purpose of this study was to assess the features of saliva crystallization in middle-aged and elderly people. The study included middle-aged (45-60 years old; n=63) and elderly (over 60 years old; n=78) men who signed an informed consent to participate. Crystalloscopic analysis of saliva in order to characterize the metabolic characteristics of the body and the composition of biological fluids. The intrinsic crystallization of mixed saliva was studied using the method of classical crystalloscopy. The structure index, crystallizability, facia destruction degree and the clearity of the marginal protein zone were determined. Each of the parameters was expressed on a 5-point scale. In general, the study made it possible to establish the presence of shifts in the crystallogenic properties of mixed saliva in the older age group (over 60 years old) relative to middle-aged people. Thus, the features of the crystalloscopic picture of biological fluid in elderly subjects included a decrease in the total crystallogenic potential of biological fluid, which was manifested in a decrease in crystallizability and the structural index of dehydrated saliva facies. In addition, the involutive shifts of the crystallograms were represented by an increase in the degree of destruction of facies and the expansion of the marginal zone of micropreparations. The revealed transformations of the crystallogenic properties of the biological medium allow us to speak about age-dependent characteristics of the component composition and physico-chemical parameters of saliva and, therefore, indirectly describe the involutive changes in the metabolome of this biological fluid

Microwave study of the effect of cold argon plasma on functional state of rat’s skin

The purpose of this study was a comparative study of the dielectric parameters of rat skin when treated with argon and argon cold plasma. The experiment was performed on 40 male Wistar rats divided into 4 equal groups. The first group of animals (n=10) was a control (intact). The rats of the remaining groups (n=10 in each) were treated daily with a pre-epilated area of the skin of the back (area = 1x1 cm). The duration of the course for all experienced groups is 10 procedures. Animals of the second group were treated with a non–ionized argon stream (the duration of one procedure was 1 minute), rats of the third and fourth groups were treated with argon cold plasma (1 and 2 minutes, respectively). Cold plasma generation was performed using a device using the principle of microwave ionization of a gas stream. Argon of high purity (99.99%) was used as the latter. The dielectric parameters of the skin of animals in the treated area (in control group rats – at a similar point in the back) were evaluated upon completion of a full course of exposure. For this purpose, a specialized software and hardware complex was used, providing near-field resonant microwave probing of biological tissues. It was found that the course treatment of the skin of the back of rats with gas streams with different ionization causes the formation of a specific functional-metabolic and morpho-structural response. Its character is determined by the parameters of the gas flow used: non-ionized argon significantly reduces the dielectric parameters (permeability and conductivity), and the result of the action of cold argon plasma depends on the exposure. During one-minute treatment, tissue permeability was observed to remain intact with a moderate decrease in conductivity. In the case of an increase in the exposure time to 2 minutes, the dielectric constant increased, and the conductivity remained unchanged

Assessment of the Action of Сold Helium Plasma on Metabolic and Physico-chemical Parameters of Rats Blood

The aim of this paper was to study the effect of cold helium plasma on the parameters of oxidative metabolism and crystallogenic activity of rat blood plasma. Material and methods: The experiment was performed on 20 male Wistar rats divided into control (n=10, without manipulation) and main (n=10) groups. Animals of the main group underwent a course, which included 10 daily procedures for processing a pre-epilated area of the back skin (area – 10% of the body surface) with a stream of helium cold plasma (duration – 1 min.). Upon completion of the course, the intensity of lipid peroxidation, total antioxidant activity, malon dialdehyde level and superoxide dismutase activity were determined in the blood of all rats, and crystallogenic properties were evaluated. The conjugation of potential shifts was carried out by correlation analysis. Results: It was found that cold helium plasma transforms oxidative metabolism of blood plasma and its crystallogenic properties, acting as an agent with moderate antioxidant properties and a stimulant of dehydration structuring. In this case, the antioxidant effect is realized, among other things, due to the activation of superoxide dismutase

Immune and metabolic response to covid-19 infection: Review for molecular pathways

The purpose of this review is a systematic analysis of data from clinical observations, international experience and reviews related to the pathogenetic aspects of the impact of a new coronavirus infection on the immune system. Information was searched in MEDLINE, PubMed, and RSCI databases. Some data for SARS-CoV-2 virus as etiological agent that provokes the development of COVID-19 are presented. Special attention was paid to immunity shifts, which were produced in patients under COVID-19 infection. The prevailing role of the “cytokine storm” in the development of severe forms of the disease is revealed in detail. Demonstrated and integrated into a single scheme of adjustment, innate and adaptive immunity is occurring with the new coronavirus infection. This information is supplemented by the characteristics of the metabolic response accompanying this pathology and by changes in the erythrocyte state under COVID-19 infection. Based on these pathogenetic mechanisms, potential variants of targeted correction of the disease are proposed and justified

Cold Argon Athmospheric Plasma for Biomedicine: Biological Effects, Applications and Possibilities

Currently, plasma medicine is a synthetic direction that unites the efforts of specialists of various profiles. For the successful formation of plasma medicine, it is necessary to solve a large complex of problems, including creating equipment for generating cold plasma, revealing the biological effects of this effect, as well as identifying and justifying the most promising areas of its application. It is known that these biological effects include antibacterial and antiviral activity, the ability to stimulate hemocoagulation, pro-regenerative properties, etc. The possibility of using the factor in tissue engineering and implantology is also shown. Based on this, the purpose of this review was to form a unified understanding of the biological effects and biomedical applications of argon cold plasma. The review shows that cold plasma, like any other physical and chemical factors, has dose dependence, and the variable parameter in this case is the exposure of its application. One of the significant characteristics determining the specificity of the cold plasma effect is the carrier gas selection. This gas carrier is not just an ionized medium but modulates the response of biosystems to it. Finally, the perception of cold plasma by cellular structures can be carried out by activating a special molecular biosensor, the functioning of which significantly depends on the parameters of the medium (in the field of plasma generation and the cell itself). Further research in this area can open up new prospects for the effective use of cold plasma

OXIDATIVE METABOLISM AND PHYSICAL PROPERTIES OF THE BLOOD IN ATHLETES

Objectives: Monitoring of metabolic status in athletes includes a wide range of different parameters, but not all of them have been fully studied. Thus, the presence of oxidative stress is noted in athletes, but its features are revealed only in fragments. The crystallogenic properties of blood in athletes have not been previously studied. Methods: The study enrolled a total of 262 athletes (19–29 years old) that are highly qualified in cyclic sports. Furthermore, screening data of apparently healthy non-exercising volunteers of the same age (n=35) were used for control. Blood serum of the athletes and non-exercising volunteers was analyzed for 8-isoprostane level, oxidized low-density lipoproteins, activity of superoxide dismutase, and glutathione peroxidase and reductase. We also studied the level α- and β-carotenes, α- and γ-tocopherols, lycopene, lutein, and zeaxanthin. Crystallogenic properties of blood serum were tested with own method. Results: The complex study demonstrated the presence of shifts in oxidative metabolism and blood serum physical and chemical properties induced by professional sports and manifested both in the status of blood pro- and antioxidative systems and in shifts of biofluids crystallogenic activity. At the same time, the obtained data confirmed the development of oxidative stress in qualified athletes. Conclusion: Our data shown that there are some shifts of oxidative metabolism and crystallogenic properties of blood plasma in professional athletes