Identification of unannotated exons of low abundance transcripts in and cloning of a new serine protease gene upregulated upon injury-0

<p><b>Copyright information:</b></p><p>Taken from "Identification of unannotated exons of low abundance transcripts in and cloning of a new serine protease gene upregulated upon injury"</p><p>http://www.biomedcentral.com/1471-2164/8/249</p><p>BMC Genomics 2007;8():249-249.</p><p>Published online 24 Jul 2007</p><p>PMCID:PMC1949825.</p><p></p>ch [16]

Identification of unannotated exons of low abundance transcripts in and cloning of a new serine protease gene upregulated upon injury-3

<p><b>Copyright information:</b></p><p>Taken from "Identification of unannotated exons of low abundance transcripts in and cloning of a new serine protease gene upregulated upon injury"</p><p>http://www.biomedcentral.com/1471-2164/8/249</p><p>BMC Genomics 2007;8():249-249.</p><p>Published online 24 Jul 2007</p><p>PMCID:PMC1949825.</p><p></p> . These sequences are from proteins that presented the highest similarity with SP212 catalytic domain sequence (e-value < -20) in searches against the NCBI databank, using Blastx. The alignment was performed using Clustalw (20). SP55, SPH144 and SP60, as well SP212, localize in the same region (88A12-B1) of chromosome 2R, whereas SP186 is located in chromosome X

Identification of unannotated exons of low abundance transcripts in and cloning of a new serine protease gene upregulated upon injury-1

<p><b>Copyright information:</b></p><p>Taken from "Identification of unannotated exons of low abundance transcripts in and cloning of a new serine protease gene upregulated upon injury"</p><p>http://www.biomedcentral.com/1471-2164/8/249</p><p>BMC Genomics 2007;8():249-249.</p><p>Published online 24 Jul 2007</p><p>PMCID:PMC1949825.</p><p></p>re hybridized to the probes indicated at the bottom of each blot. The estimated size (kb) of each transcript is indicated on the left

Identification of unannotated exons of low abundance transcripts in and cloning of a new serine protease gene upregulated upon injury-4

<p><b>Copyright information:</b></p><p>Taken from "Identification of unannotated exons of low abundance transcripts in and cloning of a new serine protease gene upregulated upon injury"</p><p>http://www.biomedcentral.com/1471-2164/8/249</p><p>BMC Genomics 2007;8():249-249.</p><p>Published online 24 Jul 2007</p><p>PMCID:PMC1949825.</p><p></p>ith a needle dipped into 10cells/ml cultures of either Gram() or Gram() bacteria or fungi (). Note that asceptic pricking by itself triggers the induction of the SP212, albeit at a lower level

Identification of unannotated exons of low abundance transcripts in and cloning of a new serine protease gene upregulated upon injury-5

<p><b>Copyright information:</b></p><p>Taken from "Identification of unannotated exons of low abundance transcripts in and cloning of a new serine protease gene upregulated upon injury"</p><p>http://www.biomedcentral.com/1471-2164/8/249</p><p>BMC Genomics 2007;8():249-249.</p><p>Published online 24 Jul 2007</p><p>PMCID:PMC1949825.</p><p></p>ch [16]

Identification of unannotated exons of low abundance transcripts in and cloning of a new serine protease gene upregulated upon injury-2

<p><b>Copyright information:</b></p><p>Taken from "Identification of unannotated exons of low abundance transcripts in and cloning of a new serine protease gene upregulated upon injury"</p><p>http://www.biomedcentral.com/1471-2164/8/249</p><p>BMC Genomics 2007;8():249-249.</p><p>Published online 24 Jul 2007</p><p>PMCID:PMC1949825.</p><p></p>imers used for cloning the SP212 cDNA. The asterisk indicates the primer that was based on the ORE-5 sequence. CG-33329 was electronically annotated. B) Sequence of the SP212 cDNA and the amino-acid sequence encoded in the largest open reading frame (GenBank accession number: ). Grey boxes mark the containing the SP catalytic residues, which are underlined