Modeling the influence of river rehabilitation scenarios on bed material sediment flux in a large river over decadal timescales

Predicted binding affinities of peptide-HLA-A*02:01 complexes involving all 9-mers from the canonical human proteome. The top panel shows the distribution of the binding affinities of all complexes, and the bottom panel shows the distribution of the binding affinities of the complexes with predicted IC50 < 500 nM. (TIF 256 kb

Gating strategy for total CD4+ and CD8+ T cells for analysis of mitochondrial membrane potential in HIV-negative and HIV+/cART individuals.

<p>(a) Lymphocytes (Circled) were defined using side scatter (SSC) and forward scatter (FSC) characteristics. (b,c) Gating strategy to identify CD3+CD4+ and CD3+CD8+ T cells within the lymphocyte population using CD3 (PE), CD4 (PE-Cy7), and CD8 (APC-Cy7) antibodies. (d,e) Comparisons of median Dioc₆ MFI in total CD4+ and CD8+ T cells between HIV+/cART and HIV-negative subjects. (f,g) Comparisons of median HE MFI in total CD4+ and CD8+ T cells between HIV+/cART and HIV-negative subjects.</p

Clinical characteristics of study population.

<p>Clinical characteristics of study population.</p

Correlations of MitoTracker red MFI against MitoTracker green and HE MFI.

<p>(a,b) The correlation of MitoTracker red MFI against the MFI of MitoTracker green and HE in total CD4+ T cells among HIV+/cART individuals. (c,d) The correlation of MitoTracker red MFI against the MFI of MitoTracker green and HE in total CD4+ T cells among HIV-negative individuals. (e,f) The correlation of MitoTracker red MFI against the MFI of HE in total CD8+ T cells among HIV+/cART and HIV-negative individuals.</p

Mitochondrial density measured by MitoTracker green MFI in total CD8+ T cells and CD8+ T cell subpopulations in HIV+/cART and HIV-negative individuals.

<p>(a,b) Median MitoTracker green MFI in CD8+ T cell subpopulations in HIV+/cART and HIV-negative individuals. (c-h) Comparisons of median MitoTracker green MFI among total and functional CD8+ T cell subpopulations between HIV+/cART and HIV-negative individuals.</p

Glut1 expression on total CD8+ T cells and CD8+ T cell subpopulations in HIV-negative and HIV+/cART individuals.

<p>(a-b) Median Glut1 MFI of functional CD8+ T cell subpopulations in HIV+/cART and HIV-negative individuals. (c-h) Comparisons of median Glut1 MFI among total and functional CD8+ T cell subpopulations between HIV+/cART and HIV-negative individuals.</p

Glut1 expression on total CD4+ T cells and CD4+ subpopulations in HIV-negative and HIV+/cART individuals.

<p>(a-b) Median percentage of CD4+Glut1+ T cells within functional CD4+ T cell subpopulations in HIV+/cART and HIV-negative individuals. (c-h) Comparisons of median percentages of CD4+Glut1+ T cells among total and functional CD4+ T cell subpopulations between HIV+/cART and HIV-negative individuals. (i-j) Correlations between percentage of CD4+Glut1+ T cells against total CD4+ T cell percentage among HIV+/cART and HIV-negative individuals.</p

Mitochondrial density measured by MitoTracker green MFI in CD4+ T cells and CD4+ T cell subpopulations in HIV+/cART and HIV-negative individuals.

<p>(a,b) Median MitoTracker green MFI in CD4+ T cell subpopulations in HIV+/cART and HIV-negative individuals. (c-h) Comparisons of median MitoTracker green MFI in CD4+ T cells among total and functional CD4+ T cell subpopulations between HIV+/cART and HIV-negative individuals.</p

Correlations of Glut1 MFI against the MFI of MitoTracker green.

<p>(a-b) Correlations of Glut1 MFI against the MFI of MitoTracker green in CD4+ T cells among HIV+/cART and HIV-negative individuals. (c) Correlation of Glut1 MFI against the MFI of MitoTracker green in CD8+ T cells among HIV-negative individuals.</p

Gating strategy to quantify Glut1-expressing cells within CD4+ and CD8+ T cell subpopulations.

<p>(a) Lymphocytes (Circled) were defined using side scatter (SSC) and forward scatter (FSC) characteristics. (b-c) Gating strategy to identify CD3+CD4+ and CD3+CD8+ T cells within the lymphocyte population. (d-e) From the CD4+ and CD8+ T cell population, 4 distinct groups were identified based on CCR7 and CD45RA surface expression. (f-i) These populations were further defined by CCR7, CD45RA and CD27 to identify N (CD27+), CM (CD27+), EM (CD27-), TM (CD27+), and TD populations (CD27-). This gating strategy has been previously described [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0183931#pone.0183931.ref023" target="_blank">23</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0183931#pone.0183931.ref024" target="_blank">24</a>].</p