3 research outputs found
Next-Generation Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Source for Mass Spectrometry Imaging and High-Throughput Screening
Infrared matrix-assisted laser desorption electrospray
ionization
(IR-MALDESI) is a hybrid, ambient ionization source that combines
the advantages of electrospray ionization and matrix-assisted laser
desorption/ionization, making it a versatile tool for both high-throughput
screening (HTS) and mass spectrometry imaging (MSI) studies. To expand
the capabilities of the IR-MALDESI source, an entirely new architecture
was designed to overcome the key limitations of the previous source.
This next-generation (NextGen) IR-MALDESI source features a vertically
mounted IR-laser, a planar translation stage with computerized sample
height control, an aluminum enclosure, and a novel mass spectrometer
interface plate. The NextGen IR-MALDESI source has improved user-friendliness,
improved overall versatility, and can be coupled to numerous Orbitrap
mass spectrometers to accommodate more research laboratories. In this
work, we highlight the benefits of the NextGen IR-MALDESI source as
an improved platform for MSI and direct analysis. We also optimize
the NextGen MALDESI source component geometries to increase target
ion abundances over a wide m/z range.
Finally, documentation is provided for each NextGen IR-MALDESI part
so that it can be replicated and incorporated into any lab space