Performing the Other: Indigenous Identity and Regional Resistance in Sonoran Literature of the 1980\u27s

During the decade of the 1980’s there occurs a preoccupation, presence and notable change in the representation of the indígena in the cultural production of the State of Sonora. This articulation of a modified ethnic discourse is a product of the economic and social changes that impacted the region during this period. Through a detailed examination of three Sonoran writers we observe how this ethnic discourse leads to a reformulation the traditional images of the indígena and reflects the negotiation between local and national interests to determine the meaning of the region, its identity and its place within the nation

Textiled Narratives: Branding, Consumption, and Mexican American Identity at the Turn of the Twentieth Century

The NaCo apparel company out of Tijuana, Mexico, created a unique line of clothing and accessories in the late 1990s and early 2000s. With its success in Mexico, the brand chose to extend its reach into the lucrative US market. The company\u27s focus on bicultural and binational images seemed a natural fit for a growing Latinx presence in the US. An analysis of the company\u27s successes and failures in the new market highlights the continued importance of borders as separators of visual economies rooted in national histories and imaginations. Additionally, their experience reveals the complexities within Latinx communities that can at once divide and unite them. This pushes back against prevalent discourses that tend toward homogenizing the political and cultural beliefs in these communities

Linear combinations of docking affinities explain quantitative differences in RTK signaling

Receptor tyrosine kinases (RTKs) process extracellular cues by activating a broad array of signaling proteins. Paradoxically, they often use the same proteins to elicit diverse and even opposing phenotypic responses. Binary, ‘on–off' wiring diagrams are therefore inadequate to explain their differences. Here, we show that when six diverse RTKs are placed in the same cellular background, they activate many of the same proteins, but to different quantitative degrees. Additionally, we find that the relative phosphorylation levels of upstream signaling proteins can be accurately predicted using linear models that rely on combinations of receptor-docking affinities and that the docking sites for phosphoinositide 3-kinase (PI3K) and Shc1 provide much of the predictive information. In contrast, we find that the phosphorylation levels of downstream proteins cannot be predicted using linear models. Taken together, these results show that information processing by RTKs can be segmented into discrete upstream and downstream steps, suggesting that the challenging task of constructing mathematical models of RTK signaling can be parsed into separate and more manageable layers

Optimization of a GCaMP calcium indicator for neural activity imaging

© The Author(s), 2012. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Journal of Neuroscience 32 (2012): 13819-13840, doi:10.1523/JNEUROSCI.2601-12.2012.Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Recent efforts in protein engineering have significantly increased the performance of GECIs. The state-of-the art single-wavelength GECI, GCaMP3, has been deployed in a number of model organisms and can reliably detect three or more action potentials in short bursts in several systems in vivo. Through protein structure determination, targeted mutagenesis, high-throughput screening, and a battery of in vitro assays, we have increased the dynamic range of GCaMP3 by severalfold, creating a family of “GCaMP5” sensors. We tested GCaMP5s in several systems: cultured neurons and astrocytes, mouse retina, and in vivo in Caenorhabditis chemosensory neurons, Drosophila larval neuromuscular junction and adult antennal lobe, zebrafish retina and tectum, and mouse visual cortex. Signal-to-noise ratio was improved by at least 2- to 3-fold. In the visual cortex, two GCaMP5 variants detected twice as many visual stimulus-responsive cells as GCaMP3. By combining in vivo imaging with electrophysiology we show that GCaMP5 fluorescence provides a more reliable measure of neuronal activity than its predecessor GCaMP3. GCaMP5 allows more sensitive detection of neural activity in vivo and may find widespread applications for cellular imaging in general.A.F. has been supported by a European Molecular Biology Organization long-term fellowship. Work in H.B.’s laboratory was funded by the National Institutes of Health (NIH) Nanomedicine Development Center “Optical Control of Biological Function,” and work in S.S.-H.W.’s laboratory was funded by NIH R01 NS045193

El Diálogo Cultural y la Transformación Genérica en El Mariachi de Robert Rodríguez

(First paragraph) El Mariachi (1993) de Robert Rodriguez ha recibido un gran número de elogios de la crítica y reconocimiento de la industria cinemática por su creatividad y su historia productiva única. Le película ganó el premio de Audience Award en el festival de cine de Sundance y posteriormente fue estrenada en video por Colombia Pictures. Desde entonces Robert Rodriguez ha llegado a ser uno de los directores latinos más destacado

A Circuit for Gradient Climbing in C. elegans Chemotaxis

Animals have a remarkable ability to track dynamic sensory information. For example, the nematode Caenorhabditis elegans can locate a diacetyl odor source across a 100,000-fold concentration range. Here, we relate neuronal properties, circuit implementation, and behavioral strategies underlying this robust navigation. Diacetyl responses in AWA olfactory neurons are concentration and history dependent; AWA integrates over time at low odor concentrations, but as concentrations rise, it desensitizes rapidly through a process requiring cilia transport. After desensitization, AWA retains sensitivity to small odor increases. The downstream AIA interneuron amplifies weak odor inputs and desensitizes further, resulting in a stereotyped response to odor increases over three orders of magnitude. The AWA-AIA circuit drives asymmetric behavioral responses to odor increases that facilitate gradient climbing. The adaptation-based circuit motif embodied by AWA and AIA shares computational properties with bacterial chemotaxis and the vertebrate retina, each providing a solution for maintaining sensitivity across a dynamic range