Improving Barrier Properties of Xylan-Coated Food Packaging Papers with Alkyl Ketene Dimer

In order to improve the hydrophobicity of xylan hemicellulose, a simple procedure of its chemical modification with alkyl ketene dimer (AKD), a non-toxic, cost-effective, and eco-friendly chemical, was performed. For this purpose, the reaction products of beech wood xylan and different amounts of hydrophobic AKD were used for paper surface treatment. Thus, the coatings of about 4.5 g/m2 were applied on both sides of base paper in single and three successive layers. To obtain a complete reaction between AKD and xylan hemicellulose, the coated papers were thermal cured (about 110 °C) and the effects of AKD content on the barrier (water, oil, and water vapours) and mechanical properties were analysed. The structural analyses by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) of coated samples emphasized the presence of β-keto-ester compounds as a result of the reaction between xylan hemicelluloses and AKD. This is confirmed by the improving of barrier properties as the AKD content in coating dispersion is higher. The good barrier performance and improved strength properties were obtained for the coated papers with xylan hemicellulose and 1% AKD applied on paper surface in three successive layers (about 4.5 g/m2). In this case, the water vapours transmission rate (WVTR) was 35% lower than those untreated and the resistance to air passing through coated papers was over 3 times higher compared with base paper. There are no results reported on the chemical reaction of xylan hemicelluloses with AKD as well as its application in coatings for paper packaging. In this context, the obtained results in this study can contribute to expand the applications area of hemicelluloses offering a sustainable strategy for the developing of food packaging papers with appropriate barrier properties using biopolymer coating materials

Green “one-pot” fluorescent bis-indolizine synthesis with whole-cell plant biocatalysis

An efficient one-pot route leading to bis-indolizine symmetric compounds has been developed via a new approach from the dipyridinium heterocyclic compound, reactive halogenated derivative, and activated alkyne through biocatalysis. A set of local plants was evaluated for its catalytic potential in “one-pot” biocatalysis of these valuable fluorescent compound synthesis reactions. Most of these biocatalysts containing enzymes from the oxidoreductase class (peroxidase: 0.56–1.08 mmol purpurogallin‧g−1 fresh weight‧min−1, polyphenol oxidase (PPO) : 27.19–48.95 PPO units‧mg tissue−1, CAT: 3.27–21.71 µmol O2‧g−1 fresh weight‧min−1), were used as green catalysts in the multi-component cycloaddition reaction, in an aqueous buffer solution, for the production of bis-indolizine compounds in moderate to excellent yields (45–85%). The horseradish root (Armoracia rusticana) has been selected as the most promising biocatalyst source among the evaluated plants, and the obtained yields were greater than in the conventional synthesis method. The structures of indolizine derivatives were confirmed by nuclear magnetic resonance spectra, elemental analyses, as well as Fourier transform-infrared spectra. The cytotoxicity of the latter obtained indolizine compounds on the growth of the model microorganism, Saccharomyces cerevisiae MIUG 3.6 yeast strain, was also evaluated. Various parameters (number of generations, growth rate, generation time, dry matter yield, the degree of the budding yeast cells, and the degree of yeast autolysis, fermentation intensity), which describe the yeast growth, suggest that the nutrient broth supplemented with different concentrations of bis-indolizine compounds (10 and 1 µM) had no toxic effect on the yeast strain growth, under submerged cultivation conditions

Different Types of Meatballs Enriched with Wild Thyme/Lemon Balm Aqueous Extract—Complex Characterization

In the context of the increasing lactation problems among breastfeeding women, the development of a healthy lifestyle is needed. Different variants of pork, turkey, and beef meatballs, with added lemon balm (Melissa officinalis L.) and wild thyme (Thymus serpyllum L.) aqueous extract (6%), were obtained. These herbs were selected and used due to their antioxidant, antimicrobial, and lactogenic potential. Two thermal treatments, hot air convection (180 °C) and steam convection (94 °C), were applied for meatballs processing. The obtained meatballs were further subjected to a complex characterization. The functionality of the plant extracts was proved by the values of total content of polyphenols (2.69 ± 0.02 mg AG/g dw) and flavonoids (3.03 ± 0.24 mg EQ/g dw). FT-IR analysis confirmed the presence of trans-anethole and estragole at 1507–1508 cm−1 and 1635–1638 cm−1, respectively. Costumers’ overall acceptance had a score above 5.5 for all samples, on a scale of 1 to 9. Further analysis and human trials should be considered regarding the use of lactogenic herbs, given their health benefits and availability

Comparative study of the chemical compositions and antioxidant activities of fresh juices from Cucurbitaceae species grown in Romania

Cucurbitaceae is a family of health-promoting plants due to their compounds with beneficial effects. The aim of this study was to analyze, for the first time, the chemical composition, the antioxidant activity and the metal chelating properties of fruit juices obtained from four different species of the Cucurbitaceae family cultivated in Romania, namely Momordica charantia, Cucumis metuliferus, Benincasa hispida and Trichosanthes cucumerina. The samples of juice were analyzed by high-performance liquid chromatography (HPLC) and all the four species displayed high levels of the two triterpenes, oleanolic and ursolic acids, and also in phenolic compounds, including catechin, (−)-epicatechin and gallic acid. The juices demonstrated significant antioxidant activity against the free radical 2,2-diphenyl-1-picrylhydrazyl (ranging from 20 to 95%,), a good iron binding ability (ranging from 7.45 ± 0.28% to 86.95 ± 0.97%) and also promising antioxidant potential against the ABTS radical (ranging from 4.97 to 32.60 μETx/mL juice). Our findings raise interesting questions for further research on Cucurbitaceae fruit juices and, consequently, their very good antioxidant potential suggests these fruits should be further explored for their protective effect against oxidative damage. This is the first time the chemical composition and antioxidant activities of fruit juices from these four Romanian Cucurbitaceae varieties have been investigated

Eco-Friendly Biosorbents Based on Microbial Biomass and Natural Polymers: Synthesis, Characterization and Application for the Removal of Drugs and Dyes from Aqueous Solutions

Pharmaceuticals and dyes are a very important part of the nonbiodegradable or hard biodegradable substances present in wastewater. Microorganisms are already known to be effective biosorbents, but the use of free microbial cells involves difficulties in their separation from effluents and limits their application in wastewater treatment. Thus, this study aimed to develop biosorbents by immobilizing Saccharomyces cerevisiae, Saccharomyces pastorianus and Saccharomyces pastorianus residual biomass on natural polymers (alginate and chitosan) and to evaluate the biosorptive potential for removal of pharmaceuticals and dyes from water. Six types of biosorbents were synthesized and characterized by Scanning Electron Microscopy and Fourier Transform Infrared Spectroscopy techniques and their biosorptive capacities for three drugs (cephalexin, rifampicin, ethacridine lactate) and two dyes (orange II and indigo carmine) were evaluated. The obtained results show that the removal efficiency depends on the polymer type used for the immobilization. In case of alginate the removal efficiency is between 40.05% and 96.41% for drugs and between 27.83% and 58.29% for dyes, while in the case of chitosan it is between 40.83% and 77.92% for drugs and between 17.17% and 44.77% for dyes. In general, the synthesized biosorbents proved to be promising for the removal of drugs and dyes from aqueous solutions

Comparative Study of Natural Antioxidants from <i>Glycine max</i>, <i>Anethum graveolensand</i> <i>Pimpinella anisum</i> Seed and Sprout Extracts Obtained by Ultrasound-Assisted Extraction

The study aimed to evaluate the antioxidant potential of sprout and seed extracts from three species of plants, namely Glycine max (GMsp-sprouts, GMsd-seeds), Anethum graveolens (AGsp-sprouts, AGsd-seeds) and Pimpinella anisum (PAsp-sprouts, PAsd-seeds), which are widely accepted by consumers and have various applications in food flavoring, and also in natural medical treatments in the pharmaceutical industries. These plants are rich in valuable compounds that show a remarkable antioxidant power and are associated with many health benefits. Ethanol extracts were obtained by ultrasound-assisted extraction and they were comparatively evaluated for their in vitro antioxidant properties. The extracts were characterized by HPTLC, HPLC-DAD, total phenol content (TPC), total flavonoid content (TFC) analysis and antioxidant activities with different assays, such as total antioxidant capacity (TAC), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation decolorization assay (ABTS), 1,1-diphenyl 1-2-picryl-hydrazyl (DPPH) and iron binding ability of chelators. Our results showed that the sprout and seed extracts of the studied plants exhibited a high content of phytochemicals and promising antioxidant properties. The highest polyphenols content was detected for AGsd (53.02 ± 0.57 mg/g DW), PAsd (48.75 ± 0.34 mg/g DW) and the highest flavonoids content for PAsp (26.84 ± 0.57 mg/g DW). Moreover, the presence of valuable compounds was demonstrated by using HPTLC, FT-IR and HPLC-DAD techniques. In order to have a better understanding of the relationship between the biological properties and the electronic structure, a molecular modelling study of genistein was also conducted. Our approach to the comparative assessment of these three plant species was based on a priori knowledge from literature data; however, this study demonstrated that these plant extracts of seeds and also sprouts are excellent sources of natural antioxidants. Significant additional differences that were found in the phytochemical composition could be exploited in future research for pharmaceutical purposes

Comparative Study of Natural Antioxidants from Glycine max, Anethum graveolensand&nbsp;Pimpinella anisum Seed and Sprout Extracts Obtained by Ultrasound-Assisted Extraction

The study aimed to evaluate the antioxidant potential of sprout and seed extracts from three species of plants, namely Glycine max (GMsp-sprouts, GMsd-seeds), Anethum graveolens (AGsp-sprouts, AGsd-seeds) and Pimpinella anisum (PAsp-sprouts, PAsd-seeds), which are widely accepted by consumers and have various applications in food flavoring, and also in natural medical treatments in the pharmaceutical industries. These plants are rich in valuable compounds that show a remarkable antioxidant power and are associated with many health benefits. Ethanol extracts were obtained by ultrasound-assisted extraction and they were comparatively evaluated for their in vitro antioxidant properties. The extracts were characterized by HPTLC, HPLC-DAD, total phenol content (TPC), total flavonoid content (TFC) analysis and antioxidant activities with different assays, such as total antioxidant capacity (TAC), 2,2&prime;-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation decolorization assay (ABTS), 1,1-diphenyl 1-2-picryl-hydrazyl (DPPH) and iron binding ability of chelators. Our results showed that the sprout and seed extracts of the studied plants exhibited a high content of phytochemicals and promising antioxidant properties. The highest polyphenols content was detected for AGsd (53.02 &plusmn; 0.57 mg/g DW), PAsd (48.75 &plusmn; 0.34 mg/g DW) and the highest flavonoids content for PAsp (26.84 &plusmn; 0.57 mg/g DW). Moreover, the presence of valuable compounds was demonstrated by using HPTLC, FT-IR and HPLC-DAD techniques. In order to have a better understanding of the relationship between the biological properties and the electronic structure, a molecular modelling study of genistein was also conducted. Our approach to the comparative assessment of these three plant species was based on a priori knowledge from literature data; however, this study demonstrated that these plant extracts of seeds and also sprouts are excellent sources of natural antioxidants. Significant additional differences that were found in the phytochemical composition could be exploited in future research for pharmaceutical purposes

The Antioxidant Potential of Ficus Umbellata Vahl (Moraceae) That Accelerates In Vitro and the In Vivo Anti-Inflammatory Protective Effects

Ficus umbellata Vahl (Moraceae), is a plant with health benefits involved in the management of menopause physiological disorders and cancers. This study aimed at investigating the antioxidant and anti-inflammatory activities of aqueous (FUAq) and methanolic (FUMeOH) extracts of Ficus umbellata. Their antioxidant activities were assayed by free radical scavenging using DPPH and ABTS assays, total antioxidant capacity, and ferrous reducing power (FRAP). Further, the effects of FUAq and FUMeOH on murine erythrocyte membrane hemolysis and protein denaturation were investigated. The in vivo anti-inflammatory activity was determined in Wistar rats with carrageenan-induced paw oedema. At tested concentrations, FUAq and FUMeOH demonstrated strong radical scavenging that was dose- and time-dependent, as well as total antioxidant capacity and ferrous ions reducing power. Moreover, they were able to stabilize murine red blood cell membranes against heat induced hemolysis and inhibit the denaturation of egg albumin at concentrations ranging from 0.125–2 mg/mL. Ficus umbellata methanolic extract at doses of 50 and 200 mg/kg endow antiedematous properties with edema inhibition percentages of 71.16 ± 1.72% and 72.98 ± 7.51%, respectively. Our findings shed light on the antioxidant and anti-inflammatory properties of Ficus umbellata that could be used in novel and safe strategies to overwhelm oxidative and inflammatory related diseases

The Antioxidant Potential of Ficus Umbellata Vahl (Moraceae) That Accelerates In Vitro and the In Vivo Anti-Inflammatory Protective Effects

Ficus umbellata Vahl (Moraceae), is a plant with health benefits involved in the management of menopause physiological disorders and cancers. This study aimed at investigating the antioxidant and anti-inflammatory activities of aqueous (FUAq) and methanolic (FUMeOH) extracts of Ficus umbellata. Their antioxidant activities were assayed by free radical scavenging using DPPH and ABTS assays, total antioxidant capacity, and ferrous reducing power (FRAP). Further, the effects of FUAq and FUMeOH on murine erythrocyte membrane hemolysis and protein denaturation were investigated. The in vivo anti-inflammatory activity was determined in Wistar rats with carrageenan-induced paw oedema. At tested concentrations, FUAq and FUMeOH demonstrated strong radical scavenging that was dose- and time-dependent, as well as total antioxidant capacity and ferrous ions reducing power. Moreover, they were able to stabilize murine red blood cell membranes against heat induced hemolysis and inhibit the denaturation of egg albumin at concentrations ranging from 0.125&ndash;2 mg/mL. Ficus umbellata methanolic extract at doses of 50 and 200 mg/kg endow antiedematous properties with edema inhibition percentages of 71.16 &plusmn; 1.72% and 72.98 &plusmn; 7.51%, respectively. Our findings shed light on the antioxidant and anti-inflammatory properties of Ficus umbellata that could be used in novel and safe strategies to overwhelm oxidative and inflammatory related diseases

Ag-Decorated Iron Oxides-Silica Magnetic Nanocomposites with Antimicrobial and Photocatalytic Activity

Nanotechnology offers unlimited possibilities for creating effective hybrid materials, which combine functional performance in environment depollution and antimicrobial defense with a lack of toxicity, biocompatibility, biodegradability, and natural availability. This paper presents the silver effect on photocatalytic and antibacterial activities of double-coated iron oxide nanoparticles (NPs), Fe3O4@SiO2/ZnO-Ag. The structural, morphological, and textural information of the, core&ndash;shell iron oxides-based superparamagnetic nanoparticles (IOMNPs) decorated with 5% Ag by ultrasound-assisted synthesis were evaluated by scanning electron microscopy with energy dispersive spectroscopy (SEM-EDX), X-ray diffraction, Raman spectroscopy, and Brunauer&ndash;Emmett&ndash;Teller physisorption measurements. Although two synthesis temperatures of 95 and 80 &deg;C were used for the co-precipitated iron oxide cores, the XRD patterns revealed the formation of a single magnetite, Fe3O4, phase. The sorption&ndash;photocatalytic activities under dark and UV irradiation encountered a maximum removal efficiency of the MB (90.47%) for the Fe3O4@SiO2/ZnO-Ag sample with iron oxide core obtained at 80 &deg;C. The rate constant for the second-order kinetics was 0.0711 min&minus;1 for 2 h, and the correlation coefficient R2 closed to unity. Two samples with Ag-decorated hybrid SiO2/ZnO shell and hierarchically interconnected porous structure with large surface area (328.8 and 342.5 m2g&minus;1) exhibited the best disk diffusion antimicrobial activity against four microorganisms, especially gram-positive Staphylococcus aureus