Features of the prion diseases associated with mutations at codon 188. Abbreviations: AA = amino acid; n.a. = not available, IHC = Immunohistochemistry; m. = month; M = methionine; V = valine; y. = years.

<p>Features of the prion diseases associated with mutations at codon 188. Abbreviations: AA = amino acid; n.a. = not available, IHC = Immunohistochemistry; m. = month; M = methionine; V = valine; y. = years.</p

Sequence analysis of <i>PRNP</i> in three patients with prion diseases.

<p>The coding region was sequenced using fluorescence-labeled primers on an automated sequencing system (LI-COR, Lincoln, Neb.). Short fragments of <i>PRNP</i> of (A) a patient with the normal codon 188, (B) patient A with the T188R mutation and (C) patient B with the T188K mutation are shown using a primer for the sequencing that reads the antisense strand.</p

Western blot analysis of PrP after proteinase K digestion (lanes 1–3) using mAb 3F4.

<p><i>Lane1:</i> PrP^Sc type 1 from a sporadic CJD case. <i>Lane 2</i>: PrP^Sc type 2 from a sporadic CJD case. <i>Lane 3</i>: Frontal cortex of patient D. As seen in lane 3 there is an additional band migrating at an apparent MW of 17 kDa.</p