O brendiranju žigova i pečata

MBP staining of murine organotypic brain slices shows myelin health status of all MOG-positive samples (MOG 1-10) and MOG-negative control (Ctrl 1) as well as healthy control sample (HC 1) in combination with human complement. (DOCX 6029 kb

Baseline clinical and demographic data and their correlation with EDSS outcome 10 years after disease onset

<p>Baseline clinical and demographic data and their correlation with EDSS outcome 10 years after disease onset</p

Variables of disease course and their correlation with EDSS outcome 10 years after disease onset

<p>Variables of disease course and their correlation with EDSS outcome 10 years after disease onset</p

time to cognitive dysfunction.

<p>Kaplan-Meier curves of the risk of developing cognitive dysfunction according to disability status 10 years after onset. Log rank test used for calculation of significancy.</p

Percentages of CD3+ T cells (A), CD19+CD138− mature B cells (B) and CD19+CD138+ plasma blasts (C) in the CSF of patients with a CIS, RRMS, CPMS and OND.

<p>Individual data points are shown as circles and horizontal bars indicate means. Data were compared using the Kruskal-Wallis test and Dunn's multiple comparison post-hoc test and overall p-values are shown in each figure. # = significant differences to the OND group.</p

Correlation of CSF B cells with other inflammatory parameters.

<p><b>1</b> data are shown as median (range), p-value: groups were compared using <b>2</b> Mann-Whitney U test or <b>3</b> Spearmans nonparametric correlations.</p><p>Abbreviations: MRI Gd+ lesions = presence of gadolinium-enhancing lesions on T1-weighted MRI (neg = negative, pos = positive), MRI T2 lesions = number of lesions on T2-weighted MRI, Q-Alb = albumin quotient, OCB = oligoclonal bands, MMP = matrix metalloproteinase, R = Spearman's correlation coefficient, ns = statistically not significant.</p

CSF B cell subsets.

<p>(A) Dot plots of CSF leucocytes according to their CD45-PerCP versus side scatter properties (left panel) and forward versus side scatter properties (right panel). Region 1 (R1, left panel) was used for acquisition of a minimum number of 1000 events and region 2 (R2, right panel) was used for analysis. CSF analysis of representative patients with a CIS (B), RRMS (C), CPMS (D) and OND (E) for the presence of CD3+ T cells and CD19+ B cells (left panel), and CD19+CD138− mature B cells, CD19+CD138+ plasma blasts and CD19−CD138+ plasma cells (right panel). The numbers represent the relative percentages of these cell populations.</p

Demographic, clinical and CSF data of analyzed patients.

<p><b>1</b> data are shown as median (range), p-value: groups were compared using <b>2</b> Qui-Square test or <b>3</b> Kruskal-Wallis test and Dunn's multiple comparison post-hoc test, # statistically significant different from OND control group.</p><p>Abbreviations: n = number of patients, y = years, EDSS = expanded disability status score, acute relapse = number of patients with an acute relapse, MRI Gd+ lesions = presence of gadolinium-enhancing lesions on T1-weighted MRI (neg = negative, pos = positive), ≥9 T2 MRI lesions = ≥9 lesions on T2-weighted MRI, OCB = oligoclonal bands, Q-Alb = albumin quotient, MMP = matrix metalloproteinase, ratio = CSF∶serum ratio, ns = statistically not significant.</p

Different staining patterns of NMO-IgG in M-1 and M-23 AQP4 transfected cells.

<p>Anti-AQP4 IgG (red) in NMO patient's serum targets AQP4 (green), which is expressed by transiently transfected HEK cells. Performing the assay for M-23 AQP4 (<b>A</b>, green) versus M-1 AQP4 (<b>B</b>, green), results in different staining patterns of NMO-IgG (red). Weaker binding was observed to M-1 AQP4, which contrary to M-23 AQP4 forms only few orthogonal arrays of particles.</p

M-1 and M-23 AQP4-IgG titer values in follow-up samples.

<p>Higher titer values of NMO-IgG in two patients with recurrent ON (1^st sample) after conversion into NMO (2^nd sample) after 2.6 and 8.7 years. With increasing M-23 AQP4-IgG titers, patient one developed Abs against full length AQP4, whereas patient two remained M-1 AQP4-IgG negative.</p