MOESM2 of Characterization of blood dendritic and regulatory T cells in asymptomatic adults with sub-microscopic Plasmodium falciparum or Plasmodium vivax infection

Additional file 2. Representative staining of DC and T cells in fresh whole blood from adults. The illustrations provided represent the flow cytometry gating strategy used to identify DC and Treg cells

Details of parasite sampling.

<p>^1–9 Superscript indicates the number of patients with missing data.</p><p>Details of parasite sampling.</p

Within-host and population diversity.

<p>Within-host and population diversity.</p

Linkage disequilibrium.

<p>Only samples with no missing data are included in the analyses. All nine loci were used in all <i>P</i>. <i>vivax</i> populations. In the <i>P</i>. <i>falciparum</i> populations, 8 loci were analysed in Bangka and Sumba, 6 in Kalimantan (exclusion of monomorphic loci TA42 and TA60), and 7 loci in West Timor (exclusion of monomorphic loci TA1).</p><p>¹ Restricted multi-locus haplotypes from samples with no more than one multi-allelic locus.</p><p>^NS Not significant (<i>P</i> > 0.05)</p><p>* <i>P</i> < 0.05</p><p>** <i>P</i> < 0.01</p><p>*** <i>P</i> < 0.001.</p><p>Linkage disequilibrium.</p

Unrooted neighbour-joining tree illustrating the genetic relatedness between <i>P</i>. <i>falciparum</i> (top) and <i>P</i>. <i>vivax</i> (bottom) isolates.

<p>Unrooted neighbour-joining tree illustrating the genetic relatedness between <i>P</i>. <i>falciparum</i> (top) and <i>P</i>. <i>vivax</i> (bottom) isolates.</p

Population structure in <i>P</i>. <i>vivax</i>.

<p>Bar plots illustrating the population structure at <i>K</i> = 2–5 in <i>P</i>. <i>vivax</i>. <i>K</i>1 = light green, <i>K</i>2 = dark green, <i>K</i>3 = red, <i>K</i>4 = orange, and <i>K</i>5 = white.</p

Age stratified prevalence of malaria diagnosed by microscopy and PCR.

<p>Microscopy (A) and microscopy and PCR (B).</p

Multiple fractional polynomial curves showing the haemoglobin concentration according to microscopic and submicroscopic parasitaemia and age.

<p>Multiple fractional polynomial curves showing the haemoglobin concentration according to microscopic and submicroscopic parasitaemia and age.</p

Malaria diagnosis and demographic features of survey participants^a

<p>Malaria diagnosis and demographic features of survey participants<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0165340#t001fn002" target="_blank">^a</a></p

Multiple fractional polynomial curves showing the probability of anaemia by microscopic and submicroscopic parasitaemia and age.

<p>Multiple fractional polynomial curves showing the probability of anaemia by microscopic and submicroscopic parasitaemia and age.</p