Additional file 1: of Phosphate uptake kinetics and tissue-specific transporter expression profiles in poplar (Populus × canescens) at different phosphorus availabilities

Table S1. In silico analyses of putative poplar phosphate transporters; Table S2. Primers used for qRT PCR of putative P transporter genes; Table S3. Transcript abundances of phosphate transporter genes; Figure S1. Biomass and performance of poplar grown with five different P concentrations; Figure S2. Neighbor-Joining tree of the amino acid sequences for inorganic phosphate transporters in poplar; Figure S3. Correlations of absolute microarray expression data (log2-value) and qRT PCR relative expression values (log2) for PtPHTs. (PDF 1255 kb

Overview on the results of General linear Models for relative nutrient element concentrations in plant cells and cell walls.

<p>Information on the model parameters is given in the text, na  =  not applicable, ns  =  not significant</p><p>Overview on the results of General linear Models for relative nutrient element concentrations in plant cells and cell walls.</p

Correlation analysis of element concentrations.

<p>Correlation analysis of element concentrations.</p

Subcellular nutrient element levels in different subcellular structures of mycorrhizal roots associated with different mycorrhizal taxa [(A) Magnesium, (B) Potassium, (C) Calcium, (D) Phosphorus, (E) Sulphur] and principle component analysis (F).

<p>Data indicate means (n≥20 to 60±SE). Different letters for a given structure (EcM) or between the different compartments (AM) indicate significant differences at P≤0.05. La  =  Lactarius subdulcis, Ce  =  Cenococcum geophilum, Cl  =  Clavulina cristata, Gl  =  Glomus sp., C  =  cell, W  =  Wall, HM  =  Hyphal mantle, HN  =  Hartig net, InterH  =  intercellular hyphae, intraH  =  intracellular hyphae, Arb  =  arbuscules, F  =  fungus, P  =  plant.</p

Cross section of beech (A, <i>Fagus sylvatica</i>) and ash (B, <i>Fraxinus excelsior</i>) mycorrhizal root tips.

<p>The beech root was colonized by <i>Lactarius subdulcis</i> and the ash root by <i>Glomus</i> sp. PCW  =  plant cell wall, HN  =  Hartig Net, PC  =  plant cell, FCW  =  fungal cell wall, HM  =  hyphal mantle, Arb  =  arbuscles, intraH =  intracellular hyphae, interH  =  intercellular hyphae. Bar = 10 µm. Area 2 (red square)  =  area for EDX-STEM mapping with 40×40 measurement points. Area 1 was used for drift correction.</p

Relative abundance of nutrient elements in plants and their associated fungal species.

<p>Nutrient elements (Mg, P, S, K, and Ca) are indicated as means across all plant structures measured and across all fungal structures measured (±SE). Different letters indicate significant differences at P<0.05 between fungal cells of different of mycorrhizal species or plant cells associated with these species. Significant differences between fungal and plant structures are indicated by bold letters for P_{(fungus/plant).}</p><p>Relative abundance of nutrient elements in plants and their associated fungal species.</p

Correlation matrix between eigengenes of co-expression modules and element concentrations and biomass.

<p>Modules were composed of genes differentially expressed between poplars (<i>P</i>. × <i>canescens</i>) grown with high P availability and plants grown with intermediate or low P availability in fine roots or leaves. Correlation coefficient and p-value (in brackets) are shown. Significant p-values (≤ 0.05) of correlation are color coded (red: negative correlation, blue: positive correlation). Numbers of DEGs in modules are shown below module’s name.</p

Dissecting nutrient-related co-expression networks in phosphate starved poplars - Fig 6

<p><b>Change in transcript abundance of P-responsive genes in roots relative to that in leaves for the modules “Green” (a), “Brown” (b) and “Blue” (c).</b> Log₂-fold change of MP/HP and LP/HP DEGs in roots were plotted to the corresponding log₂-fold changes in leaves. Abbreviations of the genes names are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171958#pone.0171958.s004" target="_blank">S3 Table</a>.</p

EDX-STEM-mapping of an ectomycorrhizal root of beech (<i>Fagus sylvatica</i>) colonized by <i>Lactarius subdulcis</i>. EDX-STEM-mapping of 40×40 measurement points in the area depicted (A) of magnesium (B, turquoise), phosphorus (C, green), sulphur (D, orange), potassium (E, purple), and calcium (F, red).

<p>Scale bar: 10 µm.</p

ANOVA results for the main factors “Treatment” and “Tissue”.

<p>ANOVA results for the main factors “Treatment” and “Tissue”.</p