Final concentration of benzo[a]pyrene (B[a]P) in culture media after treatment of the cells with various concentrations of EOM.

<p>Final concentration of benzo[a]pyrene (B[a]P) in culture media after treatment of the cells with various concentrations of EOM.</p

Relative levels of XPE, XPC and XPA mRNAs.

<p>The levels of mRNAs were analyzed after the 6 h treatment of HEL12469 cells with benzo[a]pyrene (B[a]P) and extractable organic matter (EOM) in the absence (–S9) and presence (+S9) of the microsomal S9 fraction. Mean ± SD values from three independent cell treatments are shown, asterisks denote a significant (p<0.05) increase/decrease of mRNA levels. The baseline mRNA level after treatment of the cells with DMSO is represented by a bold horizontal line.</p

Western blotting analyses of the levels of XPE, XPC and XPA proteins.

<p>Protein expression was measured after the 24 h treatment of HEL12469 cells with benzo[a]pyrene (B[a]P) and extractable organic matter (EOM) in the absence (–S9) and presence (+S9) of the microsomal S9 fraction. A representative result of two independent experiments is shown. Amido Black-stained proteins were used as a loading control.</p

PAH content in extractable organic matter (EOM) from individual samplings.

*<p>Significant differences (p<0.001) in PAH concentrations between EOMs from Prague-summer vs. Ostrava-summer and Prague-winter vs. Ostrava-winter.</p

Bulky DNA adduct levels/10⁸ nucleotides detected in DNA extracted from HEL12469 cells.

<p>The cells were treated for 24 h with benzo[a]pyrene (B[a]P) and extractable organic matter (EOM) in the absence (–S9) and presence (+S9) of the microsomal S9 fraction. P–W = Prague-winter, O–W = Ostrava-winter, P–S = Prague-summer, O–S = Ostrava-summer, N.D. – not detectable.</p

Relative levels of XPE, XPC and XPA proteins in lysates of HEL12469 cells.

<p>The cells were treated with B[a]P and EOMs for 24 h. The data represent mean protein levels relative to the control sample from two independent experiments. P–W = Prague-winter, O–W = Ostrava-winter, P–S = Prague-summer, O–S = Ostrava-summer.</p

Relative levels of unscheduled DNA synthesis (UDS).

<p>UDS in HEL12469 cells was studied after 24 h treatment with benzo[a]pyrene (B[a]P) and extractable organic matter (EOMs) in the absence (–S9) and presence (+S9) of the S9 microsomal fraction. Mean ± SD UDS values relative to the DMSO-treated control are shown, asterisks denote a significant (p<0.05) increase/decrease in the activity of UDS. The baseline levels of UDS in cells treated with DMSO is represented by a bold horizontal line. Each mean UDS value is based on the analysis of 700–2500 cells.</p