25 research outputs found
Effect on albumin and fibronectin adsorption of silver doping via ionic exchange of a silica-based bioactive glass
Protein adsorption is a crucial step in the life of biomaterials for bone application, such as bioactive glasses. The
investigation of adsorption mechanisms is a difficult task per se, which is even more complex on bioactive glasses
due to surface reactivity. Here, the effect of silver doping by ionic exchange on the interaction of a silica-based
bioactive glass with albumin and fibronectin, serum proteins related to osseointegration, is reported. The
presence of silver does not change relevant surface properties such as topography, surface energy, wettability, or
surface ζ potential. Nevertheless, the interactions with proteins are much different. The presence of silver
significantly increases the adsorption of albumin and fibronectin and leads to a higher loss of secondary structure
compared to the undoped surface, as a consequence of the interactions and bonding between silver and thiols in
the cysteine residues. Selectivity of silver-doped glass is discovered: Ag enhances more adsorption and dena-
turation of albumin since it has more cysteines than fibronectin. It is also here observed that due to the formation
of a hydrated silica gel layer during adsorption, proteins are not only present on the surface of the bioactive
glasses, but also embedded inside the surface reaction laye
Pro- and anti-oxidant properties of near-infrared (NIR) light responsive carbon nanoparticles
Elemental carbon nanomaterials (ECNMs) are redox active agents that can be exploited to purposely modify the redox balance of cells. Both pro- or antioxidant properties have been reported. However, to the best of our knowledge, there are not comprehensive studies exploring both properties on the same material in view of a potential application in medicine. At the same time, the effect of the bulk structure on the pro/antioxidant properties is poorly known. Here, carbon nanoparticles (CNPs) derived by glucose with definite size and shape have been prepared, and their redox properties evaluated in cell free systems in the dark or following activation with a Near Infrared (NIR) laser beam (945 nm, 1.3 W/cm2). We found that, when irradiated with NIR, CNPs efficiently generate heat and singlet oxygen (1O2), a property that can be exploited for dual photo-thermal (PT)/photodynamic (PD) therapy in cancer. On the other hand, in the absence of photo-activation, CNPs react with both oxidant (hydroxyl radicals) and antioxidant (glutathione) species. When tested on a murine macrophages cell line (RAW 264.7) CNPs were clearly antioxidant. Furthermore, albeit efficiently internalized, CNPs do not exert cytotoxic effect up to 80 µg/ml and do not exacerbate TNF-α-mediated inflammation. Overall, the results reported herein suggest that CNPs may represent a new class of safe nanomaterials with potential applications in medicine
Molecular Aspects of the Interaction with Gram-Negative and Gram-Positive Bacteria of Hydrothermal Carbon Nanoparticles Associated with Bac8c2,5Leu Antimicrobial Peptide
Molecular Aspects of the Interaction with Gram-Negative and Gram-
Positive Bacteria of Hydrothermal Carbon Nanoparticles Associated
with Bac8c2,5Leu Antimicrobial Peptide
Giulia Barzan,⊥ Ida Kokalari,⊥ Giacomo Gariglio, Elena Ghibaudi, Marc Devocelle, Marco P. Monopoli,
Alessio Sacco, Angelo Greco, Andrea M. Giovannozzi, Andrea M. Rossi, and Ivana Fenoglio*
Cite This: https://doi.org/10.1021/acsomega.2c00305 Read Online
ACCESS Metrics & More Article Recommendations *sı Supporting Information
ABSTRACT: Antimicrobial peptides (AMPs) are widely studied
as therapeutic agents due to their broad-spectrum efficacy against
infections. However, their clinical use is hampered by the low in
vivo bioavailability and systemic toxicity. Such limitations might be
overcome by using appropriate drug delivery systems. Here, the
preparation of a drug delivery system (DDS) by physical
conjugation of an arginine-rich peptide and hydrothermal carbon
nanoparticles (CNPs) has been explored, and its antimicrobial
efficacy against Eschericia coli (E. coli) and Staphylococcus aureus
investigated in comparison with the unloaded carrier and the free
peptide. The mechanism of interaction between CNPs and the
bacteria was investigated by scanning electron microscopy and a
combined dielectrophoresis−Raman spectroscopy method for real-
time analysis. In view of a possible systemic administration, the
effect of proteins on the stability of the DDS was investigated by using albumin as a model protein. The peptide was bounded
electrostatically to the CNPs surface, establishing an equilibrium modulated by pH and albumin. The DDS exhibited antimicrobial
activity toward the two bacterial strains, albeit lower as compared to the free peptide. The decrease in effectiveness toward E. coli was
likely due to the rapid formation of a particle-induced extracellular matrix. The present results are relevant for the future
development of hydrothermal CNPs as drug delivery agents of AMP
Machine layout and performance
The Large Hadron Collider (LHC) is one of the largest scientific instruments ever built. Since opening up a new
energy frontier for exploration in 2010, it has gathered a global user community of about 7,000 scientists working
in fundamental particle physics and the physics of hadronic matter at extreme temperature and density. To sustain
and extend its discovery potential, the LHC will need a major upgrade in the 2020s. This will increase its luminosity
(rate of collisions) by a factor of five beyond the original design value and the integrated luminosity (total
collisions created) by a factor ten. The LHC is already a highly complex and exquisitely optimised machine so this
upgrade must be carefully conceived and will require about ten years to implement. The new configuration, known
as High Luminosity LHC (HL-LHC), will rely on a number of key innovations that push accelerator technology
beyond its present limits. Among these are cutting-edge 11-12 tesla superconducting magnets, compact superconducting
cavities for beam rotation with ultra-precise phase control, new technology and physical processes
for beam collimation and 300 metre-long high-power superconducting links with negligible energy dissipation.
The present document describes the technologies and components that will be used to realise the project and is
intended to serve as the basis for the detailed engineering design of HL-LHC
Development of a candidate reference sample for the characterization of tip-enhanced Raman spectroscopy spatial resolution
Tip-Enhanced Raman Spectroscopy (TERS) imaging of self-assembled thiol monolayers' nanometric patterns
Electrochemical inactivation ofBacillusspores in drinking water using a quaternary oxide electrode
Nondestructive Raman Spectroscopy as a Tool for Early Detection and Discrimination of the Infection of Tomato Plants by Two Economically Important Viruses
Global population forecasts dictate a rapid adoption of multifaceted approaches to fulfill increasing food requirements, ameliorate food dietary value and security using sustainable and economically feasible agricultural processes. Plant pathogens induce up to 25% losses in vegetable crops and their early detection would contribute to limit their spread and economic impact. As an alternative to time-consuming, destructive, and expensive diagnostic procedures, such as immunological assays and nucleic acid-based techniques, Raman spectroscopy (RS) is a nondestructive rapid technique that generates a chemical fingerprinting of a sample, at low operating costs. Here, we assessed the suitability of RS combined to chemometric analysis to monitor the infection of an important vegetable crop plant, tomato, by two dangerous and peculiarly different viral pathogens, Tomato yellow leaf curl Sardinia virus (TYLCSV) and Tomato spotted wilt virus (TSWV). Experimentally inoculated plants were monitored over 28 days for symptom occurrence and subjected to RS analysis, alongside with measuring the virus amount by quantitative real-time PCR. RS allowed to discriminate mock inoculated (healthy) from virus-infected specimens, reaching an accuracy of >70% after only 14 days after inoculation for TYLCSV and >85% only after 8 days for TSWV, demonstrating its suitability for early detection of virus infection. Importantly, RS also highlighted spectral differences induced by the two viruses, providing specific information on the infecting agent
RAMAN spectroscopy applications in grapevine: metabolic analysis of plants infected by two different viruses
Grapevine is one of the most cultivated fruit plant among economically relevant species in the world. It is vegetatively propagated
and can be attacked by more than 80 viruses with possible detrimental effects on crop yield and wine quality. Preventive
measures relying on extensive and robust diagnosis are fundamental to guarantee the use of virus-free grapevine plants and to
manage its diseases. New phenotyping techniques for non-invasive identification of biochemical changes occurring during virus
infection can be used for rapid diagnostic purposes. Here, we have investigated the potential of Raman spectroscopy (RS) to
identify the presence of two different viruses, grapevine fan leaf virus (GFLV) and grapevine rupestris stem pitting-associated
virus (GRSPaV) in Vitis vinifera cv. Chardonnay. We showed that RS can discriminate healthy plants from those infected by each of
the two viruses, even in the absence of visible symptoms, with accuracy up to 100 and 80% for GFLV and GRSPaV, respectively.
Chemometric analyses of the Raman spectra followed by chemical measurements showed that RS could probe a decrease in the
carotenoid content in infected leaves, more profoundly altered by GFLV infection. Transcriptional analysis of genes involved in the
carotenoid pathway confirmed that this biosynthetic process is altered during infection. These results indicate that RS is a
cutting-edge alternative for a real-time dynamic monitoring of pathogens in grapevine plants and can be useful for studying the
metabolic changes ensuing from plant stresses
Direct quantification of sulfur dioxide in wine by Surface Enhanced Raman Spectroscopy
A rapid Surface Enhanced Raman Spectroscopy (SERS) method to detect SO2 in wine is presented, exploiting the preferential binding of silver nanoparticles (AgNPs) with sulfur-containing species. This interaction promotes the agglomeration of the AgNPs and inducing the formation of SERS "hot spots" responsible for SO2 signals enhancement. For increasing SO2 concentrations from 0 to100 mg/l in wine simulant, SERS intensity showed an increasing trend, following a Langmuir absorption function (R-2 = 0.94). Due to the wine matrix variability, a standard additions method was then employed for quantitative analysis in red and white wines. This method does not require the SO2 separation but only a matrix pre-cleaning by solid phase extraction. The limit of detection (LOD) was defined for each wine tested, ranging from 0.6 mg/l to 9.6 mg/l. The results obtained were validated by comparison with the International Organization of Vine and Wine method (OIV-MA-AS323-04A)