A: Magnified phylogeny of the Indian subcontinent clade.

<p>B: Magnified phylogeny of the Arctic-like 1 and 3 sub-clades. Branches are labeled with bootstrap values generated with both the neighbor-joining (1,000 bootstrap replications) and maximum likelihood (100 bootstrap replications, number in brackets) algorithms. Bootstrap values are given for selected relevant nodes only. A scale indicating genetic distance is presented by the horizontal bar. Designations of the different RABV phylogroups (clade, sub-clade and lineage) are as indicated, based on previously defined assignments <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002560#pntd.0002560-Bourhy1" target="_blank">[5]</a>, <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002560#pntd.0002560-Kuzmin1" target="_blank">[25]</a>. Isolates for which the complete N gene sequence was obtained in this study are indicated by an asterisk with the viruses from Nepal underscored.</p

Maximum clade credibility phylogenetic tree based on 67 Arctic-related RABV N gene sequences.

<p>The horizontal branches are drawn to a scale of estimated year of divergence. Upper and lower limits of the 95% highest posterior density (HPD) estimates, and the corresponding divergence dates for the selected nodes are shown. Posterior probability values are shown above the branch for relevant key nodes only. Isolates for which the complete N gene sequence was obtained in this study are indicated by an asterisk.</p

Clade specific amino acid differences in the glycoprotein of Nepalese RABV.

a<p>Amino acid positions exclude the 19aa signal peptide sequence.</p>b<p>Virus samples 3878-09 and 3878-78 have the Indian subcontinent residue.</p>c<p>Virus samples 3878-09 and 3878-78 have Thr substitution.</p>d<p>Virus sample 3878-78 has the Indian subcontinent residue.</p

Maximum likelihood phylogenetic tree based on 173 complete RABV N gene nucleotide sequences.

<p>Branches are labeled with bootstrap values generated with both the neighbor-joining (1,000 bootstrap replications) and maximum likelihood (100 bootstrap replications, number in brackets) algorithms. Bootstrap values are given for selected relevant nodes only. A scale indicating genetic distance is presented by the horizontal bar. Designations of the different RABV phylogroups (clade, sub-clade and lineage) are as indicated, based on previously defined assignments <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002560#pntd.0002560-Bourhy1" target="_blank">[5]</a>, <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002560#pntd.0002560-Kuzmin1" target="_blank">[25]</a>. Isolates for which the complete N gene sequence was obtained in this study are indicated by an asterisk with the viruses from Nepal underscored.</p

Clade specific amino acid differences in the nucleoprotein of Nepalese RABV.

a<p>Excludes virus sample 3878-10 which adopts Val at position 179.</p

Predicted signalase cleavage sites.

<p>Predicted signalase cleavage sites.</p

Characterized Australian orthobunyaviruses.

<p>Characterized Australian orthobunyaviruses.</p

Glycosylation sites of Australian orthobunyavirus M-segment polyprotein sequences.

<p>Predicted N-glycosylation sites are indicated along the M-segment sequence by their recognition sequence and aa position. MAPV, Mapputta virus; TRUV, Trubanaman virus; BUCV, Buffalo Creek virus; MURBV, Murrumbidgee virus; GGV, Gan Gan virus; SASHV, Salt Ash virus; MPKV, Maprik virus.</p

Geography of Australian Mapputta group viruses.

<p>MAPV, Mapputta virus; MPKV, Maprik virus; TRUV, Trubanaman virus; GGV, Gan Gan virus; MURBV, Murrumbidgee virus; SASHV, Salt Ash virus; BUCV, Buffalo Creek virus.</p

Phylogenetic relationship of Australian Mapputta group and K10441 isolates to other selected orthobunyaviruses.

<p>Deduced amino acid sequences of the S- (N ORF; panel <b>A</b>), M- (Gn, NSm,Gc polyprotein ORF; panel <b>B</b>), and L-segment (RdRp-ORF; panel <b>C</b>) were aligned and trees reconstructed with the Neighbor-Joining method applying a Poisson substitution model as implemented in MEGA 6. Bootstrap values resulting from 1000 pseudoreplicates are indicated at the respective nodes; scale bars indicate the number of substitutions per site, and GenBank accession number and isolate name (where known) are given next to the virus name. MAPV, Mapputta virus; MPKV, Maprik virus; TRUV, Trubanaman virus; GGV, Gan Gan virus.</p