25 research outputs found
ē¬¬1090ååčå»å¦ä¼ä¾ä¼ć»ē¬¬7åē°å¢ēå½å»å¦ē ē©¶ä¼
<p>HepG2 cells were incubated with BITC 30 Ī¼M, insulin 100 nM and insulin + BITC for 30 min. PathScan Signaling Array Kit was used for the detection of the phosphorylated AKT (Thr308). Data shown as mean of fold phosphorylation normalized to untreated control + SEM (n = 3) *p<0.05(Unpaired Studentās t test).</p
CAT gene expression in HepG2 cells modulated by BITC.
<p>Dose-dependency is shown for the antioxidant enzyme CAT. Results are presented as fold mRNA expression, normalized to the housekeeping gene RPL32 and the control. Data shown as mean value Ā± SEM *(p<0.05), **(p<0.01) and ***(p<0.001). (Oneway ANOVA and posthoc Bonferroni).</p
FOXO1 phosphorylation upon BITC treatment.
<p>HepG2 cells were incubated with 30 Ī¼M BITC for 24 h and cell lysates were prepared and subjected to Western blot analysis with antibodies for pFOXO1 Thr24 (Phospho FOXO1). Total FOXO1 was used for normalization. Data shown as mean + SEM (n = 3) ***p<0.001 (Unpaired Studentās t test).</p
HepG2 cells siRNA knock-down analyses and G6Pase gene expression.
<p>BITC 30 Ī¼M influence after starvation on G6Pase gene expression upon FOXO1, AKT, NRF2 and SIRT knock-down conditions. *p<0.05, **p<0.01 (Unpaired Studentās t test) vs control cells treated with NT ā*āor NT+BITC ā#ā, normalized with RPL32. Data shown as mean of fold mRNA + SEM (n = 4).</p
Gluconeogenic enzymes protein expression.
<p>HepG2 cells were incubated with 30 Ī¼M BITC for 24 h and cell lysates were prepared and subjected to Western immuno-blot analysis with primary antibodies for PEPCK and G6Pase. Results are normalized with GAPDH. Data shown as + SEM (n = 3) **p<0.01, ***p<0.001 (Unpaired Studentās <i>t</i> test).</p
CAT time dependency analyses in HepG2 cells modulated by BITC.
<p>Time dependent analyses for CAT. Results are presented as fold mRNA expression, normalized to the housekeeping gene RPL32 and the control. Data shown as mean value Ā± SEM *(p<0.05). (Oneway ANOVA and posthoc Bonferroni).</p
U-2 OS-FOXO1-GFP cells treated with BITC 1ā100 Ī¼M for 1h and addition of 100 nM insulin after 30 min.
<p>The curves show the relative nuclei/cytoplasm intensity of FOXO1-GFP for BITC without (black curve) and with insulin addition (red curve). Results are presented as mean values + standard error (SEM) (n = 4). Significant differences vs control (0 Ī¼M) are labelled for *(p<0.05) and **(p<0.01) (Oneway ANOVA and posthoc Dunnett T3).</p
Inhibition of U 2-OS-FOXO1-GFP cells translocation upon antioxidant protection.
<p>Stimulation with insulin 100 nM, BITC 30 Ī¼M, insulin + BITC, NAC 5 mM (N acetyl cysteine), BITC + NAC, insulin + NAC and BITC + insulin + NAC for 2 h. Data shown as mean Ā± SEM (n = 4) normalized to untreated control cells. **(p<0.01) vs BITC 30 Ī¼M (Oneway ANOVA and posthoc Dunnett T3).</p
HepG2 cells siRNA knock-down analyses and PEPCK gene expression.
<p>BITC 30 Ī¼M influence after starvation on PEPCK gene expression upon FOXO1, AKT, NRF2 and SIRT knock-down conditions. (Unpaired Studentās <i>t</i> test) vs control cells treated with NT or NT+BITC, normalized with RPL32. Data shown as mean of fold mRNA + SEM (n = 4).</p