Growth parameters of AKFV33. A, Adsorption curve; B, One step growth curve.

<p>Each curve was generated by averaging results from three independent measurements.</p

Host range and lytic capability of phage AKFV33.

a<p>Sensitivity is based on mean multiplicity of infection (MOI: the lowest ratio of phage to bacteria resulting in complete lysis of overnight bacterial culture within 5 h of incubation).</p>b<p>PTs represents phage types of STEC O157:H7.</p>c<p>ECOR collection represents standard reference strains of <i>Escherichia coli</i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034585#pone.0034585-Ochman1" target="_blank">[53]</a>.</p

AKFV33 structural proteins (Lane 2) alongside the standard marker (Lane1) separated on 10% SDS-PAGE gel and visualized by Coomassie brilliant blue R250 stain.

<p>AKFV33 structural proteins (Lane 2) alongside the standard marker (Lane1) separated on 10% SDS-PAGE gel and visualized by Coomassie brilliant blue R250 stain.</p

Transmission electron micrograph of AKFV33 negatively stained with uranyl acetate.

<p>Scale bar represents 100 nm.</p

General features of phage AKFV33 genome.

<p>General features of phage AKFV33 genome.</p

Phage AKFV33 structural proteins identified by MALDI QqTOF Mass Spectrometry.

<p>Phage AKFV33 structural proteins identified by MALDI QqTOF Mass Spectrometry.</p

Whole genome comparisons of AKFV33 and other T5-like phages using a progressive MAUVE alignment.

<p>The degree of sequence similarity is indicated by the intensity of the red region. The contiguous black boxes under the red region represent the position of the genes. A, AKFV33; B, EPS7; C, SPC35; D, T5 (ATCC11303-B5, GenBank accession#: AY587007); E: T5 (ATCC11303-B5, NC_005859).</p