Leptospirose em bovinos abatidos em matadouros no Estado do Pará.

Esta pesquisa teve como objetivo verificar a soroprevalência da leptospirose e demonstrar a presença de leptospiras em amostras de urina e rins de bovinos abatidos no matadouro da Cooperativa e Indústria Pecuária do Pará (SOCIPE), provenientes de seis municípios do Estado do Pará, Brasil, no período de agosto de 1998 a abril de 1999. Foi feita pesquisa sorológica pelo teste de soroaglutinação microscópica, utilizando-se 27 cepas de antígenos vivos para detecção de anticorpos antileptospiras, sendo a diluição máxima pesquisada de 1:800. A demonstração direta de leptospiras foram feitas pelos métodos de impregnação pela prata em amostras de rim, e de coloração de Vágó em amostras de urina e rim. Foram analisadas 131 amostras de soro, 107 de urina e 131 de rins de sete rebanhos (seis municípios), sendo que 102 (77,8%) mostraram-se positivas sorologicamente a pelo menos um sorovar e todos os rebanhos foram reagentes. À porcentagem de soropositividade nos rebanhos variou de 45 a 90%. O sorovar de maior prevalência nas amostras pesquisadas foi hebdomadis reagindo em 41,2%, seguido de hardjo com 33,6%, andamana e bratislava com 12,9% cada, patoc com 7,6% e pomona e sejroe com 6,8%. Os outros sorovares reagiram em menos de 4% cada um. O título de 800 foi encontrado nos sorovares hardjo, hebdomadis, andamana, pomona e javanica. O título de 400 foi encontrado nos sorovares djasiman, patoc e sejroe e de 200 em bratislava, castelonis, saxkoebing, shermani, pyrogenes, tarassovi e australis. Os sorovares canicola, cynopteri, wolffi, ballum, icterohaemorrhagiae e celledoni só reagiram com título de 100. Os outros sorovares não reagiram em nenhuma amostra. A demonstração direta de leptospiras pelo método de coloração de Vágó em amostras de urina mostrou 29,9% (32/107) de positividade, enquanto que em amostras de rim foi de 24,4% (32/131) de positivas. Pelo método de impregnação pela prata em amostras de rim foi possível demonstrar leptospiras em 34,7% (42/121) das amostras pesquisadas.This research was aimed to verify the seroprevalence of leptospirosis as well as to show the presence of leptospira in the slaughterhouse samples picked out at random from some counties of Pará State, Brazil. The serological research was carried out through microscopic agglutination test, using 27 strains of live antigens. The direct demonstration of leptospires through the silver impregnation method on kidney samples and through the Vágó method on urine and kidney samples was also performed. Out of the 131 samples studied from seven herds 102 (77,8%) showed positive to at least one serovar. The percentage of positivity in herds was 45 t9 90%. The serovar that showed the highest prevalence was the hebdomadis which reacted in 41,2%, followed by the hardjo in 33,6%, the andamana and bratislava in 12,9%, the patoc in 7,6%, the pomona and sejroe in 6,8%. The other serovars reacted in less than 4%. The maximum títle studied was of 800, found in the serovars hardjo, hebdomadis, andamana, pomona and the javanica. The 400 title was found in the serovars djasiman, patoc and sejroe while the 200 title was found in the bratislava, castelonis, shermani, saxcoebing, pyrogenes, tarassovi and the australis. The 100 title was found in the serovars canicola, cynopteri, wolffi, ballum, icterohaemorrhagiae e celledoni. Other serovars not reacted. at a maximum title of 100. The Vágó coloring method on urine samples for direct exposure of leptospires presented a 29,9% (32/107) positívity rate, whereas in kidney samples this rate was 24,4% (32/131). By means of the silver impregnation method on kidney samples, it was possible to detect leptospires in 34,7% (42/121) of studied samples

Clinical and serological tests for arboviruses in free-living domestic pigeons (Columba livia)

To Fapespa, for granting a Master’s scholarship, to PNMG, for capture of the birds, to the Microbiology and Immunology Laboratory of the UFRA, for collection of the samples, and to the SAARB/IEC, for their support in the development of serological tests.Universidade Federal do Pará. Instituto de Ciências Biológicas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Parque Naturalístico Mangal das Garças. Setor Veterinário. Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Belém, PA, Brasil.Universidade Federal Rural da Amazônia. Instituto da Saúde e Produção Animal. Laboratório de Biologia Molecular. Belém, PA, Brasil.Universidade Federal Rural da Amazônia. Instituto da Saúde e Produção Animal. Laboratório de Biologia Molecular. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Universidade Federal Rural da Amazônia. Instituto da Saúde e Produção Animal. Laboratório de Biologia Molecular. Belém, PA, Brasil.BACKGROUND: In this study, we evaluated the role of free-living domestic pigeons (Columba livia) as a reservoir of arboviruses in the city of Belém, state of Pará, Brazil. We investigated the presence of antibodies against the most prevalent arboviruses. OBJECTIVES: This study was aimed at evaluating some clinical and physical parameters of domestic pigeons, including the presence of antibodies to Amazon-endemic arboviruses. METHODS: Eighty-five healthy pigeons were captured in Mangal das Garças Park, in Belém, and were bled. Upon capture, the birds were subjected to a clinical examination in search of alterations that could indicate the presence of arboviruses. Blood samples were converted to serum and tested using the haemagglutination inhibition (HI) technique with a panel of 19 antigens of arboviruses circulating in the Amazon. The confirmation assay for the positive reactions to the viral species tested by HI was a neutralisation test in new-born Swiss albino mice (Mus musculus) [mouse neutralisation test (MNT)]. FINDINGS: A total of 10 (11.8%) serum samples tested positive for antiflavivirus antibodies by HI. All the samples positive for the HI test were subjected to MNT for detection of viruses and yielded negative results (logarithmic neutralisation index < 1.7). MAIN CONCLUSION: The results represent the first serological detection of antiarbovirus antibodies in domestic pigeons as potential hosts of arboviruses in Brazil. The detection of haemagglutination-inhibiting antibodies against genus Flavivirus indicated that there was recent contact between the analysed domestic pigeons and these arboviruses. Further studies are needed to evaluate the role of free-living pigeons in the maintenance cycle and spread of arboviruses in the Amazon

Clinical and serological tests for arboviruses in free-living domestic pigeons (Columba livia)

BACKGROUND In this study, we evaluated the role of free-living domestic pigeons (Columba livia) as a reservoir of arboviruses in the city of Belém, state of Pará, Brazil. We investigated the presence of antibodies against the most prevalent arboviruses. OBJECTIVES This study was aimed at evaluating some clinical and physical parameters of domestic pigeons, including the presence of antibodies to Amazon-endemic arboviruses. METHODS Eighty-five healthy pigeons were captured in Mangal das Garças Park, in Belém, and were bled. Upon capture, the birds were subjected to a clinical examination in search of alterations that could indicate the presence of arboviruses. Blood samples were converted to serum and tested using the haemagglutination inhibition (HI) technique with a panel of 19 antigens of arboviruses circulating in the Amazon. The confirmation assay for the positive reactions to the viral species tested by HI was a neutralisation test in new-born Swiss albino mice (Mus musculus) [mouse neutralisation test (MNT)]. FINDINGS A total of 10 (11.8%) serum samples tested positive for antiflavivirus antibodies by HI. All the samples positive for the HI test were subjected to MNT for detection of viruses and yielded negative results (logarithmic neutralisation index < 1.7). MAIN CONCLUSION The results represent the first serological detection of antiarbovirus antibodies in domestic pigeons as potential hosts of arboviruses in Brazil. The detection of haemagglutination-inhibiting antibodies against genus Flavivirus indicated that there was recent contact between the analysed domestic pigeons and these arboviruses. Further studies are needed to evaluate the role of free-living pigeons in the maintenance cycle and spread of arboviruses in the Amazon