28 research outputs found
Le Miroir des sports : publication hebdomadaire illustrée
01 octobre 19241924/10/01 (A14,N225)-1924/10/01
U87 cell migration.
<p>Confocal fluorescent microscopy images of platinum nanoparticles (NP-Pt) or cisplatin -treated and untreated U87 glioma cells. (A) U87 control; (B) NP-Pt—treated U87; (C) cisplatin-treated U87; (D) effect of NP-Pt on the migration of U87 glioma cells <sup>a, b</sup> Values with different letters are significantly different, P < 0.05. Bar scale 40μm. Note: red arrows—U87 glioma cells shown as an overlaid image of 4′,6-diamidino-2-phenylindole-stained nuclei (blue). Abbreviations: C—control group.</p
Protein expression level of PCNA at U87 glioma tumour tissue.
<p>Visualization of PCNA <i>via</i> immunohistochemistry (A, B, C) and immunofluorescence (D, E, F, G, H, I) in glioblastoma tumours. (A), (D), (G)- U87 control; (B), (E), (H)- NP-Pt—treated U87; (C), (F), (I)- cisplatin-treated U87. Scale bars: 50 μm. Note: white arrows point to PCNA positive nuclei.</p
Cell morphology and BrdU incorporation assay.
<p>Optical microscopy images of platinum nanoparticles (NP-Pt) or cisplatin -treated and untreated U87 and U118 glioma cells. (A) U87 control; (B) NP-Pt—treated U87; (C) cisplatin-treated U87; (D) U118 control; (E) NP-Pt-treated U118; (F) cisplatin-treated U118. BrdU incorporation assay–(G)–U87 cells; (H)–U188 cells. <sup>a, b</sup> Values with different letters are significantly different, P < 0.05. Bar scale 100μm.</p
Formation of 8-HO-dG in nuclear DNA of human gliblastoma multiforme tumour tissue cells treated with platinum nanoparticles and cisplatin.
<p>Formation of 8-HO-dG in nuclear DNA of human gliblastoma multiforme tumour tissue cells treated with platinum nanoparticles and cisplatin.</p
Transmission electron microscopic images of platinum nanoparticles.
<p>Bar scale 100nm.</p
Numbers of PCNA positive nuclei in glioblastoma multiforme tumour tissue (area counted 3500 ÎĽm<sup>2</sup>) in the control group and in groups treated with platinum nanoparticles and cisplatin.
<p>Numbers of PCNA positive nuclei in glioblastoma multiforme tumour tissue (area counted 3500 ÎĽm<sup>2</sup>) in the control group and in groups treated with platinum nanoparticles and cisplatin.</p
Glioblastoma and hepatoma cells status (CI).
<p>U87, U87-EGFP, C3A and C3A-EGFP CI monitored by a real-time cell analyzer (RTCA) after 1 and 2 h of exposure to the culture medium containing graphite and diamond nanoparticles at different concentrations: diamond 20 μg/mL (ND 20), 50 μg/mL (ND 50) and 100 μg/mL (ND 100), and graphite 20 μg/mL (NG 20), 50 μg/mL (NG 50) and 100 μg/mL (NG 100); nontreated cells were used as a control (NT). (<b>A</b>) U87 (blue lines) and C3A cells (red lines) treated with graphite (NG) after 1 h (vertical blue marker line) and 2 h (vertical red marker line) and control (vertical green marker line); (<b>B</b>) U87-EGFP cells (violet lines) and C3A-EGFP cells (green lines) treated with graphite after 1 h (vertical blue marker line) and 2 h (vertical red marker line) and control (vertical green marker line); (<b>C</b>) U87 (blue lines) and C3A cells (red lines) treated with diamond (ND) after 1 h (vertical blue marker line) and 2 h (vertical red marker line) and control (vertical green marker line); (<b>D</b>) U87-EGFP cells (violet lines) and C3A-EGFP cells (green lines) treated with diamond (ND) after 1 h (vertical blue marker line) and 2 h (vertical red marker line) and control (vertical green marker line). Data analysis was carried out by two-way ANOVA and the differences between the groups were tested by Duncan’s test. Data were averaged from three replicates (n = 3); <i>P</i>< 0.05. No significant interaction (nanoparticles with transduction) was observed.</p
Concentration of C-reactive protein (CRP) in rat liver (ng/mg of liver tissue).
<p>Results presented as mean ± standard deviation (n = 3).* The value significantly (<i>P</i>< 0.05) differs from the placebo group.</p
Transmission electron microscopy images of nanoparticles.
<p>Images of (<b>A</b>) diamond (ND) and (<b>B</b>) graphite (NG) nanoparticles. Scale bar: 100 nm.</p