The Volume Fraction of a Non-overlapping Germ-grain Model

We discuss the volume fraction of a model of non--overlapping convex grains. It is obtained from thinning a Poisson process where each point has a weight and is the centre of a grain, by removing any grain that is overlapped by one of larger or equal weight. In the limit as the intensity of the Poisson process tends to infinity, the model can be identified with the intact grains in the dead leaves model if the weights are independent of the grain sizes. In this case we can show that the volume fraction is at most $1/2^d$ for $d=1$ or $2$ if the shape is fixed, but the size and the orientation are random. The upper bound is achieved for centrally symmetric sets of the same size and orientation. For general $d$ we can show the upper bound, $1/2^d$, for spherical grains with two--point radius distribution. If dependence between weight and size is allowed, it is possible to achieve a volume fraction arbitrarily close to one

Characteristics of crashes involving injured children in side impacts

The objective of this study was to define the crash characteristics of near-side impact crashes in which children seated in the rear rows are injured. The crash characteristics included the direction of force, heading angle, horizontal impact location, vertical impact location, extent of deformation and intrusion at the child occupant's seating position. Cases from in-depth crash investigation databases of the NASS-CDS (National Automotive Sampling System-Crashworthiness Data System), CIREN (Crash Injury Research and Engineering Network) and Chalmers University of Technology were reviewed. The principal direction of force was most frequently between 60° and 75°. The heading angle of the bullet vehicle was most commonly between 61° and 90°. The bullet vehicle hit the passenger compartment of the target vehicle, particularly the rear door. Often, one or both of the adjacent pillars to the rear door were involved, most commonly the B pillar. In 11 of 16 crashes, the car sill was not engaged. Most commonly, the deformation extent was into Zone 3 or more – about 40 cm – and the intrusion at the child's seating position was in the range 20–30 cm. This review of the crashes revealed differences between the current side impact test procedures and the actual side impact crashes in which children were injured

Global Tumor RNA Expression in Early Establishment of Experimental Tumor Growth and Related Angiogenesis following Cox-Inhibition Evaluated by Microarray Analysis

Altered expression of COX-2 and overproduction of prostaglandins, particularly prostaglandin E2, are common in malignant tumors. Consequently, non-steroidal anti-inflammatory drugs (NSAIDs) attenuate tumor net growth, tumor related cachexia, improve appetite and prolong survival. We have also reported that COX-inhibition (indomethacin) interfered with early onset of tumor endothelial cell growth, tumor cell proliferation and apoptosis. It is however still unclear whether such effects are restricted to metabolic alterations closely related to eicosanoid pathways and corresponding regulators, or whether a whole variety of gene products are involved both up- and downstream effects of eicosanoids. Therefore, present experiments were performed by the use of an in vivo, intravital chamber technique, where micro-tumor growth and related angiogenesis were analyzed by microarray to evaluate for changes in global RNA expression caused by indomethacin treatment

COX-2 gene expression in colon cancer tissue related to regulating factors and promoter methylation status

<p>Abstract</p> <p>Background</p> <p>Increased cyclooxygenase activity promotes progression of colorectal cancer, but the mechanisms behind COX-2 induction remain elusive. This study was therefore aimed to define external cell signaling and transcription factors relating to high COX-2 expression in colon cancer tissue.</p> <p>Method</p> <p>Tumor and normal colon tissue were collected at primary curative operation in 48 unselected patients. COX-2 expression in tumor and normal colon tissue was quantified including microarray analyses on tumor mRNA accounting for high and low tumor COX-2 expression. Cross hybridization was performed between tumor and normal colon tissue. Methylation status of up-stream COX-2 promoter region was evaluated.</p> <p>Results</p> <p>Tumors with high COX-2 expression displayed large differences in gene expression compared to normal colon. Numerous genes with altered expression appeared in tumors of high COX-2 expression compared to tumors of low COX-2. COX-2 expression in normal colon was increased in patients with tumors of high COX-2 compared to normal colon from patients with tumors of low COX-2. IL1β, IL6 and iNOS transcripts were up-regulated among external cell signaling factors; nine transcription factors (ATF3, C/EBP, c-Fos, Fos-B, JDP2, JunB, c-Maf, NF-κB, TCF4) showed increased expression and 5 (AP-2, CBP, Elk-1, p53, PEA3) were decreased in tumors with high COX-2. The promoter region of COX-2 gene did not show consistent methylation in tumor or normal colon tissue.</p> <p>Conclusions</p> <p>Transcription and external cell signaling factors are altered as covariates to COX-2 expression in colon cancer tissue, but DNA methylation of the COX-2 promoter region was not a significant factor behind COX-2 expression in tumor and normal colon tissue.</p

In Situ Visualization of the Structural Evolution and Alignment of Lyotropic Liquid Crystals in Confined Flow

Self‐assembled materials such as lyotropic liquid crystals offer a wide variety of structures and applications by tuning the composition. Understanding materials behavior under flow and the induced alignment is wanted in order to tailor structure related properties. A method to visualize the structure and anisotropy of ordered systems in situ under dynamic conditions is presented where flow‐induced nanostructural alignment in microfluidic channels is observed by scanning small angle X‐ray scattering in hexagonal and lamellar self‐assembled phases. In the hexagonal phase, the material in regions with high extensional flow exhibits orientation perpendicular to the flow and is oriented in the flow direction only in regions with a high enough shear rate. For the lamellar phase, a flow‐induced morphological transition occurs from aligned lamellae toward multilamellar vesicles. However, the vesicles do not withstand the mechanical forces and break in extended lamellae in regions with high shear rates. This evolution of nanostructure with different shear rates can be correlated with a shear thinning viscosity curve with different slopes. The results demonstrate new fundamental knowledge about the structuring of liquid crystals under flow. The methodology widens the quantitative investigation of complex structures and identifies important mechanisms of reorientation and structural changes

Performance of a 70-mer oligonucleotide microarray for genotyping of Campylobacter jejuni

<p>Abstract</p> <p>Background</p> <p><it>Campylobacter jejuni </it>is widespread in the environment and is the major cause of bacterial gastroenteritis in humans. In the present study we use microarray-based comparative genomic hybridizations (CGH), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) to analyze closely related <it>C. jejuni </it>isolates from chicken and human infection.</p> <p>Results</p> <p>With the exception of one isolate, the microarray data clusters the isolates according to the five groups determined by PFGE. In contrast, MLST defines only three genotypes among the isolates, indicating a lower resolution. All methods show that there is no inherit difference between isolates infecting humans and chicken, suggesting a common underlying population of <it>C. jejuni</it>. We further identify regions that frequently differ between isolates, including both previously described and novel regions. Finally, we show that genes that belong to certain functional groups differ between isolates more often than expected by chance.</p> <p>Conclusion</p> <p>In this study we demonstrated the utility of 70-mer oligonucleotide microarrays for genotyping of <it>Campylobacter jejuni </it>isolates, with resolution outperforming MLST.</p

Nanostructure and anisotropy of 3D printed lyotropic liquid crystals studied by scattering and birefringence imaging

Extrusion-based 3D printing of hexagonal and lamellar lyotropic liquid crystals is a powerful technique to produce hierarchical materials with well-defined anisotropic structure. Tailoring the properties of 3D printed objects requires a precise control of the nanostructure; however, a sufficiently high degree of anisotropy is often not achieved. In this study, scanning small angle X-ray scattering was performed\ua0in situ\ua0at the exit of the needle during 3D printing. We study the induced anisotropy and nanostructure in hexagonal and lamellar lyotropic liquid crystals. Mapping of extruded filaments during printing revealed that narrower nozzle diameters (370 μm) resulted in less anisotropic structures with a wider distribution of orientation angles across the cross section, while larger nozzle diameters (550 μm) resulted in more anisotropic structures with an overall higher degree of orientation. The wall shear rate is higher for the narrower nozzle, which produces wall slip, resulting in a highly anisotropic shell, and a less aligned filament core. Further examination of the filaments revealed phase transitions due to solvent evaporation. The time scales were of 10 – 20\ua0min of exposure to atmospheric conditions. Simultaneously, a loss in the macroscopic anisotropy of the hexagonal self-assembled structure was observed. These processes occur during and after extrusion-based 3D printing of liquid crystals and limit the fine control of the final structure. The variability of structures achieved for our different systems highlights the importance of structural characterization during and after extrusion to guarantee high anisotropy and well-defined structures

Incidence of early posterior shoulder dislocation in brachial plexus birth palsy

<p>Abstract</p> <p>Background</p> <p>Posterior dislocation of the shoulder in brachial plexus birth palsy during the first year of life is rare but the incidence increases with age. The aim was to calculate the incidence of these lesions in children below one year of age.</p> <p>Methods</p> <p>The incidence of brachial plexus birth lesion and occurrence of posterior shoulder dislocation was calculated based on a prospective follow up of all brachial plexus patients at an age below one in Malmö municipality, Sweden, 2000–2005.</p> <p>Results</p> <p>The incidence of brachial plexus birth palsy was 3.8/1000 living infants and year with a corresponding incidence of posterior shoulder dislocation (history, clinical examination and x-ray) during the first year of 0.28/1000 living infants and year, i.e. 7.3% of all brachial plexus birth palsies.</p> <p>Conclusion</p> <p>All children with a brachial plexus birth lesion (incidence 3.8‰) should be screened, above the assessment of neurological recovery, during the first year of life for posterior dislocation of the shoulder (incidence 0.28‰) since such a condition may occur in 7% of children with a brachial plexus birth lesion.</p

National monitoring of iodine, sodium, and vitamin D status in toddlers and women of childbearing age - results and lessons learned from a pilot study in Norway

BACKGROUND Norway is lacking a population-based national monitoring program for iodine, sodium, and vitamin D status. OBJECTIVE The aim of this study was to pilot-test a study design for collecting biological samples from a country-representative sample of 2-year-old children and their mothers and to report results for iodine, salt, and vitamin D at baseline, before initiation of salt iodization in Norway. DESIGN In a cross-sectional study, we recruited 2-year-old children and their mothers during the routine 2-year check-up through 38 randomly selected health clinics in 2021. Spot urine samples were analyzed for iodine, creatinine, and sodium, and dried blood spots from the mothers were analyzed for thyroglobulin (Tg) and 25-hydroxyvitamin D (25(OH)D). RESULTS We aimed at including 400 mother-child pairs but recruited only 55 pairs. Major challenges were closed health clinics due to the COVID-19 pandemic, lack of motivation of the health personnel to prioritize recruiting, missing information about non-participation, and high workload for participants. The median urinary iodine concentration (UIC) was 123 (95% CI: 76, 228) µg/L in the toddlers and 83 (95% CI: 72, 99) µg/L in the mothers. The median urinary sodium concentration (UNaC) was 62 (95% CI: 37, 91) mmol/L in the toddlers and 93 (95% CI: 77, 107) mmol/L in the mothers. Of the mothers, 18% had levels of 25(OH)D <50 nmol/L (suboptimal status). DISCUSSION AND CONCLUSION Lessons learned from the pilot study will be used to design a national monitoring program for toddlers and women of childbearing age in Norway. The results indicate that 2-year-old children and women of childbearing age in Norway may have inadequate iodine intakes at the group level, while for vitamin D, most of the mothers had adequate status

REAR SEAT SAFETY IN FRONTAL TO SIDE IMPACTS – FOCUSING ON OCCUPANTS FROM 3YRS TO SMALL ADULTS

ABSTRACT This study presents a broad comprehensive research effort that combines expertise from industry and academia and uses various methodologies with applied research directed towards countermeasures. The project includes real world crash data analysis, real world driving studies and crash testing and simulations, aiming at enhancing the safety of forward facing child occupants (aged 3y to small adults) in the rear seat during frontal to side impacts. The real world crash data analyses of properly restrained children originate from European as well as US data. Frontal and side impact crash tests are analyzed using different sizes of crash test dummies in different sitting postures. Side impact parameter studies using FE-models are run. The sitting posture and behavior of 12 children are monitored while riding in the rear seat. Also, the body kinematics and belt position during actual braking and turning maneuvers are studied for 16 rear seat child occupants and for various child dummies. Real world crash data indicates that several of the injured children in frontal impacts, despite being properly restrained, impacted the vehicle interior structure with their head/face resulting in serious injury. This was attributed to oblique crashes, pre-crash vehicle maneuvers or high crash severity. Crash tests confirm the importance of proper initial belt-fit for best protection. The crash tests also highlight the difficulty in obtaining the real world kinematics and head impact locations using existing crashtest dummies and test procedures. The side impact parameter studies indicate that the vehicle’s occupant protection systems, such as airbags and seat belt pretensioners, play an important role in protecting children as well. The results from the on-road driving studies illustrate the variation of sitting postures during riding in the rear seat giving valuable input to the effects of the restraint systems and to how representative the standardized dummy seating positioning procedures are. The results from the maneuver driving studies illustrate the importance of understanding the kinematics of a child relative to the seat belt in a real world maneuver situation. Real world safety of rear seat occupants, especially children, involves evaluation of protection beyond standard crash testing scenarios in frontal and side impact conditions. This project explores the complete context of rear seat protection in impact situations ranging from front to side and directions in between highlighting the importance of pre-crash posture and behavior. This research project at SAFER (Vehicle and Traffic Safety Centre at Chalmers), where researchers from the industry and universities cooperate with the aim to further improve safety for children (from 3y) to small adults in the rear seat, speeds up the process to safety implementation due to the interaction between academic and industrial researchers