Early specification of sensory neuron fate revealed by expression and function of neurogenins in the chick embryo

The generation of sensory and autonomic neurons from the neural crest requires the functions of two classes of basic helix-loop-helix (bHLH) transcription factors, the Neurogenins (NGNs) and MASH-1, respectively (Fode, C., Gradwohl, G., Morin, X., Dierich, A., LeMeur, M., Goridis, C. and Guillemot, F. (1998) Neuron 20, 483-494; Guillemot, F., Lo, L.-C., Johnson, J. E., Auerbach, A., Anderson, D. J. and Joyner, A. L. (1993) Cell 75, 463-476; Ma, Q., Chen, Z. F., Barrantes, I. B., de la Pompa, J. L. and Anderson, D. J. (1998 Neuron 20, 469-482). We have cloned two chick NGNs and found that they are expressed in a subset of neural crest cells early in their migration. Ectopic expression of the NGNs in vivo biases migrating neural crest cells to localize in the sensory ganglia, and induces the expression of sensory neuron-appropriate markers in non-sensory crest derivatives. Surprisingly, the NGNs can also induce the expression of multiple pan-neuronal and sensory-specific markers in the dermomyotome, a mesodermal derivative. Taken together, these data suggest that a subset of neural crest cells may already be specified for a sensory neuron fate early in migration, as a consequence of NGN expression

Relevance of single nephron studies to human glomerular function

Over the past decades, the search for clues to the understanding of renal function and disease has led to the ongoing development of innovative laboratory techniques to approach these questions on the single nephron level. Development of renal micropuncture techniques has allowed assessment of the single nephron glomerular filtration (SNGFR) and its determinants, as well as of tubular transport characteristics. The history and techniques of micropuncture study have recently been reviewed [1]. This brief review will focus on three specific aspects of how single nephron studies have been used to provide insights into human glomerular function: explication of hemodynamic patterns of disease; definition of effects of hormonal mediators of glomerular function, allowing correlation with hemodynamic disease patterns; and identification of mechanisms contributing to efficacy of therapeutic interventions

Anne Bronte and the religious novel in the early nineteenth century

To the introspective, sensitive, religious individual, the opening years of the 19th century must have indeed appeared to be a “Fire-Whirlwind” in which destruction in creation were simultaneous and immediate. It was an era of religious transition and uncertainty. The traditional religious beliefs and values were rapidly crumbling, in new currents of thought proliferated. Writing in 1884, J. A. Froude recalled that the first half of the century had been “an era of new ideas, of swift if silent spiritual revolution…. All were agreed to have done with compromise and conventionalities…. The present generation which has grown up in an open spiritual ocean and has learned to swim for itself will never know what it was to find the lights all drifting , the compass all awry, and nothing left to steer but by the stars.

A Reliability Study on the Self-Report Behavioral Measure for Evaluating Therapeutic Outcome

Because the original reliability study using the Self-Report Behavioral Measure for Evaluating Therapeutic Outcomes (Behavioral Checklist) used college students as subjects, and since the target population for use with this instrument is a client population, there is a need for a reliability study using clients in treatment as subjects. The objective of this study was to assess the reliability of the Behavioral Checklist using a client population. The secondary objective was to revise the Behavioral Checklist, if necessary, in order to meet the standards of reliability for testing instruments. Three reliability measures were implemented in order to evaluate and revise the Behavioral Checklist. An item analysis and split-half reliability analysis were conducted after one administration of Elliott\u27s Behavioral Checklist using a client population in treatment at a mental health center. Since these methods are measures of internal consistency, the statistical analyses were used to revise the instrument, eliminating unnecessary items and simplifying instructions based on the statistical analysis. The revised Behavioral Checklist was then administered to two subject populations (clients at a mental health center and people on probation) using the test-retest model for evaluating reliability. The test-retest analysis resulted in correlations of .889 for the subject population drawn from a mental health center and .899 for t he subject population drawn from probationers. The current study did, in fact, improve the Behavioral Checklist, making it easy to administer, and demonstrated that it is a reliable instrument for use with a client population

Exploring associations between perceived HCV status and injecting risk behaviors among recent initiates to injecting drug use in Glasgow

The aim of this study was to explore the influence of testing for hepatitis C virus (HCV) and perceived HCV status on injecting risk behavior. A cross-sectional, community-wide survey was undertaken at multiple sites throughout Greater Glasgow during 2001-2002. Four hundred ninety-seven injecting drug users (IDUs) consented to participate and were interviewed using a structured questionnaire to ascertain HCV test history and injecting risk behavior. The average age of participants was 27 years and the majority of the sample were male (70.4%). Participants had been injecting for an average duration of 2.5 years. Logistic regression analysis revealed no significant associations between having been tested and injecting risk behavior. After adjustment for potential confounding variables, HCV-negatives were significantly less likely to borrow needles/syringes and spoons or filters as compared with unawares and were significantly less likely to borrow spoons or filters as compared with HCV-positives. Due to the cross-sectional design of the study, it is uncertain whether this reduction in risk behavior could be attributed to perception of HCV status. Further research is recommended to consolidate the evidence for this relationship