2 research outputs found
Construction of a 4 Zeptoliters Switchable 3D DNA Box Origami
The DNA origami technique is a recently developed self-assembly method that allows construction of 3D objects at the nanoscale for various applications. In the current study we report the production of a 18 × 18 × 24 nm<sup>3</sup> hollow DNA box origami structure with a switchable lid. The structure was efficiently produced and characterized by atomic force microscopy, transmission electron microscopy, and Förster resonance energy transfer spectroscopy. The DNA box has a unique reclosing mechanism, which enables it to repeatedly open and close in response to a unique set of DNA keys. This DNA device can potentially be used for a broad range of applications such as controlling the function of single molecules, controlled drug delivery, and molecular computing
Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum
Antigen recognition
by antibodies plays an important role in human
biology and in the development of diseases. This interaction provides
a basis for multiple diagnostic assays and is a guide for treatments.
We have developed dihydropyridine-based fluorophores that form stable
complexes with double-stranded DNA and upon recognition of the antibodies
to DNA (anti-DNA) provide an optical response. The fluorophores described
herein have advantageous optical properties compared to those of the
currently available dyes making them valuable for research and clinical
diagnostics. By studying a series of novel fluorophores, crucial parameters
for the design were established, providing the required sensitivity
and specificity in the detection of antibodies. Using these DNA–fluorophore
complexes in a direct immunofluorescence assay, antibodies to DNA
are specifically detected in 80 patients diagnosed with an autoimmune
disease, systemic lupus erythematosus. Positivity indicated by emission
change of α-(4′-<i>O</i>-methoxyphenyl)-2-furyl
dihydropyridine strongly correlates with other disease biomarkers
and autoimmune arthritis