5 research outputs found
GO analysis of regulated proteins by LPS.
<p>(A) Upregulated proteins by LPS treatment belonged to the GO classes: respiratory electron transport chain and generation of precursor metabolites and energy processes. (B) Downregulated proteins especially belonged to the GO class protein glycosylation and to a smaller degree lipid metabolic processes. (C and D) Schematic overview of the distribution of upregulated (C) and downregulated (D) proteins in different GO classes represented here by gene name. Abbreviations: please see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0159747#pone.0159747.s001" target="_blank">S1 Table</a>.</p
STAT1 expression measured by qPCR and SILAC-MS.
<p>(A) STAT1 gene expression measured by qPCR in 3T3-L1 cells incubated with LPS and resveratrol. (B) IFIT1 protein expression measured by SILAC-MS in 3T3-L1 cells incubated with LPS and resveratrol.</p
Differentiation of 3T3-L1 cells.
<p>Lipoprotein lipase (lpl) expression during the differentiation process of 3T3-L1 cells.</p
IFIT1 expression in whole adipose tissue measured by qPCR and in 3T3-L1 cells measured by and SILAC-MS.
<p>(A) Mice treated with LPS for 28 days showed increased gene expression of IFIT1, which was ameliorated by resveratrol delivered through the diet. (B) IFIT1 protein expression measured by SILAC-MS in 3T3-L1 cells incubated with LPS and resveratrol.</p