Regional neuroinflammation in AD subjects and controls measured with [³H]PK11195 autoradiography.

<div><p>A. Representative autoradiographic and anatomical images. </p> <p>Top –Hippocampal anatomy is shown on the left followed by a representative pseudocolored TSPO autoradiogram from hippocampus of a control subject and a representative autoradiogram from hippocampus of a subject with AD. The bottom row depicts striatal anatomy on the left followed by a representative TSPO autoradiogram from a control striatum and a representative autoradiogram from a subject with AD. </p> <p>Autoradiograms were pseudocolored using the rainbow spectrum (bar on the right).</p> <p>B. Quantitative autoradiographic measurements of regional TSPO density (expressed as specific binding of [3H]PK11195 in nCi/mg tissue).</p> <p>Abbreviations: CA1-4 cornu ammoni fields of the hippocampus, DG=gentate gyrus, SubP=subpyramidal layers of CA1, Sub=subiculum, ECx=entorhinal cortex, Str=striatum. </p> <p>*p<0.05, **p<0.005 AD relative to control; ANOVA followed by Fisher’s PLSD posthoc test. </p> <p>C. TSPO density in CA1 pyramidal cell body layer as a function of Braak stage (left) and genotype (right). </p> <p>*p<0.05 relative to Braak stages 0-II, one way ANOVA followed by Fisher’s PLSD posthoc test.</p> <p>**p<0.005, student’s t test. </p></div

Regional TSPO mRNA gene expression in relation to AD pathology and ApoE genotype.

<div><p>A. TSPO gene expression by region and disease state. TSPO mRNA gene expression was measured by RT-PCR and expressed as fold change over expression in the reference sample. **p<0.005.</p> <p>B. TSPO gene expression as a function of Braak stage in hippocampus (left) and striatum (right).</p> <p>*p<0.05 relative to Braak stages 0-II, one way ANOVA followed by Fisher’s PLSD posthoc test.</p> <p>C. Effect of genotype on TSPO gene expression in hippocampus (left) and striatum (right).</p> <p>*p<0.05, students t-test 2 tailed.</p></div

Region dependent correlation between TSPO and NMDAR density.

<div><p>Quantitative analyses of [³H]MK801 binding to NMDAR and [³H]PK11195 binding to TSPO were performed by autoradiography. The correlation between NMDAR and TSPO density was negative in the CA1 hippocampal field (left, <i>n</i> = 38, Spearman’s <i>p</i> < 0.001) and positive in the striatum (right, <i>n</i> = 37, Spearman’s <i>p</i> < 0.001).</p> <p>SB=specific binding.</p></div

Regional NMDA receptor density in AD and control subjects measured with [³H]MK801 autoradiography.

<div><p>A. Representative autoradiographic and anatomical images. </p> <p>Top –Hippocampal anatomy is shown on the left followed by a representative pseudocolored NMDAR autoradiogram from hippocampus of a control subject and a representative autoradiogram from hippocampus of a subject with AD. The bottom row depicts striatal anatomy on the left followed by a representative NMDAR autoradiogram from a control striatum and a representative autoradiogram from a subject with AD. Autoradiograms were pseudocolored using the rainbow spectrum (bar on the right).</p> <p>B. Quantitative autoradiographic measurements of regional NMDAR density (expressed as specific binding of [³H]MK801 in nCi/mg tissue).</p> <p>Abbreviations: CA1-4 cornu ammoni fields of the hippocampus, DG=gentate gyrus, SubP=subpyramidal layers of CA1(including stratum radiatum, lacunosum, and moleculare),, Sub=subiculum, ECx=entorhinal cortex, Str=striatum. </p> <p>*p<0.05, **p<0.005 AD relative to control; ANOVA followed by Fisher’s PLSD posthoc test. </p> <p>C. NMDAR density in the CA1 pyramidal layer as a function of Braak stage (left) and genotype (right). </p> <p>*p<0.05 relative to Braak stages 0-II, one way ANOVA followed by Fisher’s PLSD posthoc test.</p> <p>**p<0.005, student’s t test. </p></div

sj-docx-2-mde-10.1177_23821205231203783 - Supplemental material for Design, Implementation, and Evaluation of an Intensive Course on Issues in Women's Health and Gender-Based Medicine

Supplemental material, sj-docx-2-mde-10.1177_23821205231203783 for Design, Implementation, and Evaluation of an Intensive Course on Issues in Women's Health and Gender-Based Medicine by Maricedes Acosta-Martínez, Latha Chandran, Siobhan Cohen and Anat Biegon in Journal of Medical Education and Curricular Development</p

sj-docx-1-mde-10.1177_23821205231203783 - Supplemental material for Design, Implementation, and Evaluation of an Intensive Course on Issues in Women's Health and Gender-Based Medicine

Supplemental material, sj-docx-1-mde-10.1177_23821205231203783 for Design, Implementation, and Evaluation of an Intensive Course on Issues in Women's Health and Gender-Based Medicine by Maricedes Acosta-Martínez, Latha Chandran, Siobhan Cohen and Anat Biegon in Journal of Medical Education and Curricular Development</p

sj-docx-3-mde-10.1177_23821205231203783 - Supplemental material for Design, Implementation, and Evaluation of an Intensive Course on Issues in Women's Health and Gender-Based Medicine

Supplemental material, sj-docx-3-mde-10.1177_23821205231203783 for Design, Implementation, and Evaluation of an Intensive Course on Issues in Women's Health and Gender-Based Medicine by Maricedes Acosta-Martínez, Latha Chandran, Siobhan Cohen and Anat Biegon in Journal of Medical Education and Curricular Development</p