2 research outputs found
Perturbation of Lipid Bilayers by Biomimetic Photoswitches Based on Cyclocurcumin
The use of photoswitches which may be activated by suitable
electromagnetic
radiation is an attractive alternative to conventional photodynamic
therapy. Here, we report all-atom molecular dynamics simulation of
a biomimetic photoswitch derived from cyclocurcumin and experiencing E/Z photoisomerization. In particular,
we show that the two isomers interact persistently with a lipid bilayer
modeling a cellular membrane. Furthermore, the interaction with the
membrane is strongly dependent on the concentration, and a transition
between ordered and disordered arrangements of the photoswitches is
observed. We also confirm that the structural parameters of the bilayer
are differently affected by the two isomers and hence can be modulated
through photoswitching, offering interesting perspectives for future
applications
Biomimetic Photo-Switches Softening Model Lipid Membranes
We report the synthesis and characterization of a novel
photo-switch
based on biomimetic cyclocurcumin analogous and interacting with the
lipid bilayer, which can be used in the framework of oxygen-independent
light-induced therapy. More specifically, by using molecular dynamics
simulations and free energy techniques, we show that the inclusion
of hydrophobic substituents is needed to allow insertion in the lipid
membrane. After having confirmed experimentally that the substituents
do not preclude the efficient photoisomerization, we show through
UV–vis and dynamic light scattering measurements together with
compression isotherms that the chromophore is internalized in both
lipid vesicles and monomolecular film, respectively, inducing their
fluidification. The irradiation of the chromophore-loaded lipid aggregates
modifies their properties due to the different organization of the
two diastereoisomers, E and Z. In
particular, a competition between a fast structural reorganization
and a slower expulsion of the chromophore after isomerization can
be observed in the kinetic profiles recorded during E to Z photoisomerization. This report paves the
way for future investigations in the optimization of biomimetic photoswitches
potentially useful in modern light-induced therapeutic strategies