Average cell viability and functional status according to the MTT assay in conjunctival cells incubated with different PGA dilutions for different times.

<p>NC: negative controls for cytotoxicity; PC: positive controls for cytotoxicity.</p

Average cell viability and functional status according to the WST-1 assay in conjunctival cells incubated with different PGA dilutions for different times.

<p>NC: negative controls for cytotoxicity; PC: positive controls for cytotoxicity.</p

Average cell viability and functional status according to the LIVE/DEAD assay in conjunctival cells incubated with different PGA dilutions for different times.

<p>NC: negative controls for cytotoxicity; PC: positive controls for cytotoxicity.</p

Illustrative images corresponding to the analysis of cell viability and functional status according to the LIVE/DEAD assay in conjunctival cells incubated with different PGAs dilution for different times.

<p>A: negative controls for cytotoxicity; B: positive controls for cytotoxicity; C: cells incubated with a 25% concentration of bimatoprost for 1 h; D: cells incubated with a 25% concentration of tafluprost for 1 h; E: cells incubated with a 25% concentration of travoprost for 1 h; F: cells incubated with a 25% concentration of latanoprost for 1 h. Scale bars: 100 μm.</p

Average lethal dose 50 (LD50) for each prostaglandin analog and each assay.

<p>Average lethal dose 50 (LD50) for each prostaglandin analog and each assay.</p