Histochemical and immunohistochemical analysis of Dupuytren's diseased contracture cords (DDC), palmar fascia clinically unaffected by Dupuytren's disease contracture (NPF), and normal forehand fascia (NFF).

<p>3A: Detection of glycoproteins by PAS staining. 3B: Laminin staining by immunohistochemistry. 3C: Quantification of proteoglycans by alcian blue staining. 3D: Aggrecan staining by immunohistochemistry. 3E: Decorin staining by immunohistochemistry. 3F: Versican staining by immunohistochemistry.</p

Recultivation and cell proliferation analyses of Dupuytren's diseased contracture cords (DDC), palmar fascia clinically unaffected by Dupuytren's disease contracture (NPF), and normal forehand fascia (NFF).

<p>The top panel shows representative images of cells cultured from each tissue type after 7, 14 and 21 days of culture, and the lowest panel shows the analysis of cell proliferation using PCNA immunohistochemistry with two magnification levels.</p

Quantification of the cell number and contents of key ECM components in each tissue type.

<p>NPF: palmar fascia non affected by Dupuytren's disease contracture; DDC: Dupuytren's disease contracture cords; NFF: normal forehand fascia. Values correspond to average ± standard error. Cell number: quantification of the number of cells per area of tissue; SMA: smooth muscle actin. For each variable, the statistical p value for the global comparison using the Kruskal-Wallis test and for the one-to-one comparisons using the Mann-Whitney test are shown.</p><p>Quantification of the cell number and contents of key ECM components in each tissue type.</p

Analysis of the extracellular matrix fibrillar components of Dupuytren's diseased contracture cords (DDC), palmar fascia clinically unaffected by Dupuytren's disease contracture (NPF), and normal forehand fascia (NFF).

<p>2A: Identification of collagen fibers as determined by picrosirius staining. 2B: Analysis of orientation of collagen fibers as determined by picrosirius staining using polarized microscopy. 2C: Staining of reticular fibers by using the technique of Gomori. 2D: Analysis of elastic fibers as determined by orcein staining.</p

Microarray expression of ECM components and relevant WNT-pathway genes in the three tissue types analyzed in this work.

<p>Each gene was classified as a fibrillar ECM component (fibers), glycosaminoglycan (GAG), glycoprotein or proteoglycan. NPF: palmar fascia non affected by Dupuytren's disease contracture; DDC: Dupuytren's disease contracture cords; NFF: normal forehand fascia. Statistically significant differences for the U-rank test are labeled with asterisks: in the NPF column, asterisks show statistically significant differences for the comparison of NPF vs. DDC samples; in the DDC column, for the comparison DDC vs. NFF; in the NFF column, for the NFF vs. NPF comparison.</p><p>Microarray expression of ECM components and relevant WNT-pathway genes in the three tissue types analyzed in this work.</p

Unsupervised hierarchical cluster analysis of the different samples included in the present study.

<p>Overexpressed genes are shown in green and downregulated genes are shown in red. The classification tree of the samples is displayed at the right side of the figure. N: normal forehand fascia (NFF); Palm: palmar fascia clinically unaffected by Dupuytren's disease contracture (NPF); Dupuy: Dupuytren's diseased contracture cords (DDC).</p