Caracterización de la autocompatibilidad de variedades locales de cerezo mediante cruzamientos y análisis genético: abstract

Póste

Genetic diversity and self-incompatibility studies in sweet cherry

Food and Agriculture (FA COST Action FA1104: Sustainable production of high-quality cherries for the European market

Other self-compatible sweet cherries varieties from Spain

Food and Agriculture (FA COST Action FA1104: Sustainable production of high-quality cherries for the European market

Caracterización de la autoincompatibilidad en material autóctono de cerezo

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S-locus diversity of sweet cherry varieties from Galicia, North Western Spain (abstract)

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Characterization of accessions of ‘Reine Claude Verte’ plum using Prunus SRR and phenotypic traits

European plum (Prunus domestica L.) cv ‘Reine Claude Verte’ is highly appreciated for its excellent organoleptic qualities. Despite its increasing demand, this cultivar is in the process of being eradicated in many commercial orchards because of its generally erratic fruit setting. This situation led us to explore the behaviour of particular accessions with the aim of evaluating if some of them can crop more regularly. For this purpose, we initially evaluated the putative diversity of 24 European plum accessions of ‘Reine Claude Verte’ collected in different areas of Spain. For the molecular characterization, 16 Prunus SSRs were used. Eight of them were selected from previous works of SSRs transferability within the genus and the other eight were selected from a transferability screening of another 75 Prunus SSRs, in which 65 SSRs were conserved in the species. Morphological characterization was performed by evaluating 33 qualitative characters of leaves and fruits. Results allowed differentiating the accessions by their genotypic profile and their phenotype. The accessions were grouped in six genotypic profiles according to their genetic similarity and in seven clusters according to their fruit and leaf traits. High similarity was found between the ‘Reine Claude Verte’ commercial cultivars. Some of the prospected accessions sharing the same genotypic profile showed some morphological differences, whereas some accessions with different genotypic profiles presented fruit traits similar to ‘Reine Claude Verte’. The combined use of molecular and morphological characterization allowed identifying different clones of the cultivar that were selected for further agronomic evaluation.Peer ReviewedPublishe

Genetic Dissection of Bloom Time in Low Chilling Sweet Cherry (Prunus avium L.) Using a Multi-Family QTL Approach

Bloom time in sweet cherry (Prunus avium L.) is a highly heritable trait that varies between genotypes and depends on the environmental conditions. Bud-break occurs after chill and heat requirements of each genotype are fulfilled, and dormancy is released. Bloom time is a critical trait for fruit production as matching cultivar adaptation to the growing area is essential for adequate fruit set. Additionally, low chilling cultivars are of interest to extend sweet cherry production to warmer regions, and for the crop adaptation to increasing winter and spring temperatures. The aim of this work is to investigate the genetic control of this trait by analyzing multiple families derived from the low chilling and extra-early flowering local Spanish cultivar ‘Cristobalina’ and other cultivars with higher chilling requirements and medium to late bloom times. Bloom time evaluation in six related sweet cherry populations confirmed a high heritability of this trait, and skewed distribution toward late flowering, revealing possible dominance of the late bloom alleles. SNP genotyping of the six populations (n = 406) resulted in a consensus map of 1269 SNPs. Quantitative trait loci (QTL) analysis using the Bayesian approach implemented by FlexQTL™ software revealed two major QTLs on linkage groups 1 and 2 (qP-BT1.1m and qP-BT2.1m) that explained 47.6% of the phenotypic variation. The QTL on linkage group 1 was mapped to a 0.26 Mbp region that overlaps with the DORMANCY ASSOCIATED MADS-BOX (DAM) genes. This finding is consistent with peach results that indicate that these genes are major determinants of chilling requirement in Prunus. Haplotype analysis of the linkage group 1 and 2 QTL regions showed that ‘Cristobalina’ was the only cultivar tested that contributed early bloom time alleles for these two QTLs. This work contributes to knowledge of the genetic control of chilling requirement and bloom date and will enable marker-assisted selection for low chilling in sweet cherry breeding programs

Differential expression of cherry MYB10 in white and red varieties is responsible for anthocyanin levels [abstract]

PublishedPoster number 5

Next generation equencing analysis to identify modifier gene candidates conferring pollen-parts self-compatibility in sweet cherry ‘Cristobalina’ (abstract)

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Correlación y análisis genético de maduración y calidad de fruto en cerezo

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