SLEV RT-PCR positive mosquito pools collected in Córdoba city, Argentina between 2001 and 2004.

<p>SLEV RT-PCR positive mosquito pools collected in Córdoba city, Argentina between 2001 and 2004.</p

SLEV genotype activity in mosquitoes collected in Córdoba city between 2001–2004.

<p><b>a</b>) SLEV genotypes temporal distribution. <b>b</b>) SLEV genotypes geographical distribution. LAU: Libre del Ambiente University; BOT: Botanic Garden; GUI: Guiñazú; PH: Pediatric Hospital; BG: Bajo Grande; MS: Military School; SA: San Antonio; SC: San Carlos. White circles represent sampled sites without SLEV activity detected during the study period.</p

Phylogenetic Neighbor Joining analysis of SLEV.

<p>In bold strains detected in mosquitoes during our study in Córdoba, Argentina. Percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (10 000 replicates) is shown next to the branches. The scale bar indicates substitutions per site. </p

Mosquito collection carried out in Córdoba during summer and fall season between 2001–2004.

<p>Mosquito collection carried out in Córdoba during summer and fall season between 2001–2004.</p

Sampling sites where mosquito collections were carried out during 2001–2004.

<p>LAU: Libre del Ambiente University; BOT: Botanic Garden; GUI: Guiñazú; PH: Pediatric Hospital; BG: Bajo Grande; MS: Military School; SA: San Antonio; SC: San Carlos. </p

Rarefaction curves of the number of host species detected in relation to the number of female mosquito blood meals analysed.

<p>Rarefaction curves of the number of host species detected in relation to the number of female mosquito blood meals analysed.</p

Origin of blood meals of the five mosquito species analysed in the three different wetlands in SW Spain.

<p>Only the main bird and mammal species are shown. Numbers above bars indicate the number of bloodmeals per mosquito species obtained for each sampled wetland. Percentage of blood meals for each vertebrate species/group are give as numbers inside the bars when higher than 5%. Only species/wetlands with more than 10 bloodmeals were included in the figure.</p

WNV transmission risks for each different mosquito species estimated from the 12 sampling sites for birds, horses and humans.

<p>For localities with the same estimates dots have been slightly displaced horizontally to avoid complete symbol overlapping. Solid circles indicate those mosquito species and sampling sites in which WNV has been reported in mosquitoes (Vázquez et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039549#pone.0039549-Vzquez1" target="_blank">[20]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039549#pone.0039549-Vzquez2" target="_blank">[21]</a>). Estimates are reported for 4 localities for <i>Cx. modestus</i>, 4 for <i>Cx. perexiguus</i>, 5 for <i>Cx. pipiens</i>, 10 for <i>Cx. theileri</i>, and 10 for <i>Oc. caspius</i>. One was added to all estimates to allow plotting on a log10 scale.</p

Explained variance and statistical significance of mosquito species (Species), Wetland, Locality, Season and Year in relation to the presence of bird, mammal, human and horse blood in female mosquitoes.

<p>Explained variance and statistical significance of mosquito species (Species), Wetland, Locality, Season and Year in relation to the presence of bird, mammal, human and horse blood in female mosquitoes.</p

Origin of the blood meals identified in five different mosquito species during 2007–2009.

<p>The number of blood meals on horses and humans are also included in the mammal data.</p