Additional file 1: of PPARG2 Pro12Ala polymorphism influences body composition changes in severely obese patients consuming extra virgin olive oil: a randomized clinical trial

Table S1. Changes in the body composition and metabolic parameters after 12 weeks of nutritional intervention. (DOCX 66 kb

Additional file 3: of Predicting progression of mild cognitive impairment to dementia using neuropsychological data: a supervised learning approach using time windows

Replication of the methodology proposed in previous works [28, 29] with the data(CCC) used in our study. (DOCX 121 kb

Additional file 1: of Predicting progression of mild cognitive impairment to dementia using neuropsychological data: a supervised learning approach using time windows

Table describing the cognitive domains tested by each neuropsychological data of the sample. (DOCX 21 kb

Real time PCR primer sequences of the genes studied.

<p>B2M – β2-microglobulin; PMM1 - phosphomannomutase 1; IL1B – interleukin-1β; IL6 – interleukin-6; TNF – tumor necrosis factor; TGFB1 – transforming growth factor β1; BMP – Bone morphogenetic protein; IGF1 – insulin growth factor-1; FGF2 – fibroblast growth factor 2; PDGFB – platelet derived growth factor β; OPG – osteoprotegerin; RANK – receptor activator of nuclear factor κB; RANKL – RANK ligand; CBFA1/RUNX2 – core binding factor α1/runt-related transcription factor 2; OSX – osterix; ALP – alkaline phosphatase; SOST – sclerostin; TRAP – tartrate-resistant acid phosphatase; CTSK – cathepsin K; ITGB3 – subunit β3 of the integrin αvβ3; ATP - ATPase H⁺ transporter.</p

Comparison between the relative gene expression levels of patients submitted to hip replacement surgery due to low-energy fracture in relation to the days between fracture and surgery.

<p>Values represent median (Q1–Q3).</p><p>Comparisons between the 3 groups performed with Kruskall-Wallis H test.</p><p>*p-value for comparison between the 3 groups.</p><p>IL1B – interleukin-1β; IL6 – interleukin-6; TNF – tumor necrosis factor; TGFB1 – transforming growth factor β1; BMP – Bone morphogenetic protein; IGF1 – insulin growth factor-1; FGF2 – fibroblast growth factor 2; PDGFB – platelet derived growth factor β; OPG – osteoprotegerin; RANK – receptor activator of nuclear factor κB; RANKL – RANK ligand; CBFA1/RUNX2 – core binding factor α1/runt-related transcription factor 2; OSX – osterix; ALP – alkaline phosphatase; SOST – sclerostin; TRAP – tartrate-resistant acid phosphatase; CTSK – cathepsin K; ITGB3 – subunit β3 of the integrin αvβ3; ATP - ATPase H⁺ transporter.</p

Relative expression of inflammation and growth factors genes grouped according to the time between the event of fracture and the surgery.

<p>Each gene was normalized to the expression of the housekeeping genes <i>B2M</i> and <i>PMM1</i>. *p-value<0.05 for comparisons between the three groups. (Points represent median values). IL1B – interleukin-1β; IL6 – interleukin-6; TNF – tumor necrosis factor; IGF1 – insulin growth factor-1 ; FGF2 – fibroblast growth factor 2 ; PDGFB – platelet derived growth factor β; BMP – Bone morphogenetic protein; TGFB1 – transforming growth factor β1.</p

Relative expression of bone metabolism-related genes divided according to the time between the event of fracture and the surgery.

<p><i>RANK</i>, <i>RANKL</i> and <i>OPG</i> (<b>A</b>), osteoblast (<b>B</b>), osteocyte (<b>C</b>) and osteoclast-specific genes (<b>D</b>) were studied in the three study groups. Each gene was normalized to the expression of the housekeeping genes <i>B2M</i> and <i>PMM1</i>. *p-value<0.05 for comparisons between the three groups. (Points represent median values). RANK – receptor activator of nuclear factor κB; RANKL – RANK ligand; OPG – osteoprotegerin; CBFA1/RUNX2 – core binding factor α1/runt-related transcription factor 2; OSX – osterix; ALP – alkaline phosphatase; SOST – sclerostin; ITGB3 – subunit β3 of the integrin αvβ3; TRAP – tartrate-resistant acid phosphatase; ATP - ATPase H⁺ transporter; CTSK – cathepsin K.</p

Description of the study population divided between the event of fracture and surgery.

<p>Values represent mean±standard deviation.</p><p>Comparisons between groups performed with ANOVA or chi-square tests.</p

Influenza cases reported to the national influenza surveillance system by each participating country (from southern- to northern-most) and percentages of cases due to influenza type B virus.

<p>Influenza cases reported to the national influenza surveillance system by each participating country (from southern- to northern-most) and percentages of cases due to influenza type B virus.</p

Mean percentage of influenza cases by month (black diamonds) and number of times the peak of the influenza season took place in each month (pink squares) for countries in the inter-tropical belt.

<p>Mean percentage of influenza cases by month (black diamonds) and number of times the peak of the influenza season took place in each month (pink squares) for countries in the inter-tropical belt.</p