AgB8 protein species identified in sQS_f and bQS_f by 2-DGE followed by MALDI-TOF/TOF.

<p>AgB8 protein species identified in sQS_f and bQS_f by 2-DGE followed by MALDI-TOF/TOF.</p

AgB8 protein species identified in sQS_f and bQS_f by LC-MS/MS.

<p>AgB8 protein species identified in sQS_f and bQS_f by LC-MS/MS.</p

Composition of native AgB: sQS_f apolipoproteins identification by 2-DGE plus MALDI-TOF/TOF and sLd_f lipid moiety analysis.

<p><b>A)</b> Analysis of sQS_f by 2-DGE (Figure is representative of analytical triplicates), using a 3–10 lineal gradient of pH in the first dimension, and a 15% polyacrylamide gel for SDS-PAGE in the second dimension. Gels were stained with colloidal coomassie. The presence of host and parasite components was studied by analysing all spots by MS (MALDI-TOF/TOF). AgB was found in spots regularly spaced at around 8, 16 and 24 kDa (bold circles and arrows). The table illustrates which AgB8 subunits were identified in spots corresponding to the monomeric, dimeric and trimeric forms of AgB. MW: molecular weight (KDa). <b>B)</b> Analysis of sLd_f by HPTLC (Figure is representative of analytical triplicates). Standards and samples (about 10 μg) were applied onto HPTLC plates and resolving using double development solvent system for characterisation of both neutral and polar lipid classes. Lipid bands were visualised using iodine vapour. Std: standard containing polar and neutral lipids; PC: phosphatidylcholine; PS: phosphatidylserine; PI: phosphatidylinositol; CLP: cardiolipin and PE: phsophatidylethanolamine; CHO: cholesterol; FA: free fatty acids; TAG: triacylglycerols; FAMEs: fatty acid methyl esters; SE: sterol esters.</p