Gene tree for capuchin monkeys using Dataset 3

<p>Gene tree using the cytochrome b for capuchin monkey species using Dataset 3. For more information see Nascimento et al. Journal of Biogeography (under review).</p

Gene tree for capuchin monkey using Dataset 1

<p>Gene tree using the cytochrome b for capuchin monkey species using Dataset 1. For more information see Nascimento et al. Journal of Biogeography (under review).</p

Species tree for capuchin monkeys using Dataset 3

<p>Species tree generated using Dataset 3. For more information please see Nascimento et al. Journal of Biogeography (Accepted for publication).</p

Gene tree for capuchin monkeys using Dataset 2

<p>Gene tree using the cytochrome b for capuchin monkey species using Dataset 2. For more information see Nascimento et al. Journal of Biogeography (under review).</p

BEAST input file for Dataset 1

<p>BEAST input file for Bayesian phylogenetic analysis for capuchin monkey species that simultaneously estimates the gene tree, the species tree and phylogeographic reconstructions. For more information regarding the different Datasets see Nascimento et al. Journal of Biogeography (under review).</p

All files

<p>All files used in paper Nascimento et al. 2015 (42:1349–1357) Reanalysis of the biogeographical hypothesis of range expansion between robust and gracile capuchin monkeys. Journal of Biogeography (DOI: 10.1111/jbi.12448).</p

DNA sequence alignment for capuchin monkeys using Dataset 3

<p>DNA sequence alignment in nexus format for the cytochrome b using Dataset 3. For more information see Nascimento et al. Journal of Biogeography (under review).</p

DNA sequence alignment for capuchin monkeys using Dataset 2

<p>DNA sequence alignment in nexus format for the cytochrome b gene using Dataset 2</p

DNA sequence alignment for capuchin monkeys using Dataset 1

<p>DNA sequence alignment in nexus format for the cytochrome b using Dataset 1. For more information see Nascimento et al. Journal of Biogeography (under review).</p

Involvement of Potato (<i>Solanum tuberosum</i> L.) MKK6 in Response to <i>Potato virus Y</i>

<div><p>Mitogen-activated protein kinase (MAPK) cascades have crucial roles in the regulation of plant development and in plant responses to stress. Plant recognition of pathogen-associated molecular patterns or pathogen-derived effector proteins has been shown to trigger activation of several MAPKs. This then controls defence responses, including synthesis and/or signalling of defence hormones and activation of defence related genes. The MAPK cascade genes are highly complex and interconnected, and thus the precise signalling mechanisms in specific plant–pathogen interactions are still not known. Here we investigated the MAPK signalling network involved in immune responses of potato (<i>Solanum tuberosum</i> L.) to <i>Potato virus Y</i>, an important potato pathogen worldwide. Sequence analysis was performed to identify the complete MAPK kinase (MKK) family in potato, and to identify those regulated in the hypersensitive resistance response to <i>Potato virus Y</i> infection. <i>Arabidopsis</i> has 10 MKK family members, of which we identified five in potato and tomato (<i>Solanum lycopersicum</i> L.), and eight in <i>Nicotiana benthamiana</i>. Among these, St<i>MKK6</i> is the most strongly regulated gene in response to <i>Potato virus Y</i>. The salicylic acid treatment revealed that St<i>MKK6</i> is regulated by the hormone that is in agreement with the salicylic acid-regulated domains found in the St<i>MKK6</i> promoter. The involvement of St<i>MKK6</i> in potato defence response was confirmed by localisation studies, where StMKK6 accumulated strongly only in <i>Potato-virus-Y</i>-infected plants, and predominantly in the cell nucleus. Using a yeast two-hybrid method, we identified three StMKK6 targets downstream in the MAPK cascade: StMAPK4_2, StMAPK6 and StMAPK13. These data together provide further insight into the StMKK6 signalling module and its involvement in plant defence.</p></div