Effect of CALHM1 mutations on Ca²⁺ influx.

<p><b>A</b>. HEK-293 cells transfected with GFP, CALHM1-WT, CALHM1-P86L, CALHM1-R154H, CALHM1-A213T and CALHM1-G330D were loaded with Fura-2 and tested for Ca²⁺ influx into the cytoplasm following the removal of Ca²⁺ from the extracellular media. Time course of Ca²⁺ signals normalized to the value before the addition of Ca²⁺ and expressed as the mean±S.E.M. are shown. <b>B</b>. Mean peak Ca²⁺ signals for the different conditions are shown. Number of cells analysed are given for each condition. * <i>P</i><0.05 <i>vs</i> GFP; ** <i>P</i><0.01 <i>vs</i> GFP, one-way ANOVA and Bonferroni <i>post </i><i>hoc</i>.</p

Effect of CALHM1 on <b>Aβ</b>_1-42- induced cell toxicity.

<p>HEK-293 cells over-expressing CALHM1-WT, CALHM1-G330D and CALHM1-P86L were treated with 2 μM Aβ_1-42 oligomers for 24h. Cell viability was measured by MTT reduction. Data are the mean ± SEM of three independent experiments performed in triplicate. * <i>P</i><0.05 vs untreated cells by student t test.</p