Inverse PCR sequences

<p>AACA4F-AACA4RI (REACTION AACA4F): An inverse PCR reaction was performed with primers AACAF and AACA4RI and the sequencing reaction was performed with primer AACA4F. This sequencing revealed the excised, circular form with the configuration “aacA4-gcu14”</p> <p>AACA4F-AACA4RI (REACTION AACA4RI): An inverse PCR reaction was performed with primers AACAF and AACA4RI and the sequencing reaction was performed with primer AACA4RI. This sequencing revealed the excised, circular form with the configuration “aacA4-gcu14”</p> <p>GESFSQ-GESR (REACTION GESFSQ): An inverse PCR reaction was performed with primers GESFSQ and GESR and the sequencing reaction was performed with primer GESFSQ. This sequencing revealed the excised, circular form with the full length configuration “gcu14-GES-1/aacA4”.<br>GESFSQ-GESR (REACTION GESR): An inverse PCR reaction was performed with primers GESFSQ and GESR and the sequencing reaction was performed with primer GESR. This sequencing revealed the excised, circular form with the full length configuration “gcu14-GES-1/aacA4”.<br>GESFSQ-GESR (REACTION AACA4F): An inverse PCR reaction was performed with primers GESFSQ and GESR and the sequencing reaction was performed with primer AACA4F. This sequencing revealed the excised, circular form with the full length configuration “gcu14-GES-1/aacA4”.<br>GESFSQ-GESR (REACTION AACA4FI): An inverse PCR reaction was performed with primers GESFSQ and GESR and the sequencing reaction was performed with primer AACA4FI. This sequencing revealed the excised, circular form with the full length configuration “gcu14-GES-1/aacA4”.<br>GESFSQ-GESR (REACTION AACA4R): An inverse PCR reaction was performed with primers GESFSQ and GESR and the sequencing reaction was performed with primer AACA4R. This sequencing revealed the excised, circular form with the full length configuration “gcu14-GES-1/aacA4”.<br>Orf126 menor: An inverse PCR reaction was performed with primers GCU FSQ and GCU RSQ and the sequencing reaction was performed with primer GCU FSQ. This sequencing revealed the excised, circular form with the configuration “gcu14”.</p> <p> </p

RQ fused cassette raw data for Pseudomonas aeruginosa isolates PS1 and PS26

<p>The first column describes the P. aeruginosa isolates (PS1 and PS26) and the gene targeted in the quantitative PCR for measuring their transcription (gcu14; GES-1; aacA4; and the fusion GES-1-aacA4 and rpsL), which was performed in triplicate. Also in this column are the negative controls for each PCR reaction (NTC). The second column displays the cycle threshold (CT), i.e., the PCR cycle in which the fluorescence was detected by the machine. The third and the fourth columns refer to the standard deviation and the average of CT values, respectively, between triplicates. The fifth column refers to the to the normalization of the target gene transcript amount relative to that of endogenous gene (rpsL). The sixth column corresponds to the results of relative quantification. For example, the gcu14 gene and GES gene in PS1 had RQ values of 15,88 and 7,80. It means that gcu14 is 2-fold more transcribed than GES in PS1 isolate.</p

Phylogenetic relationships between <i>Trypanosoma cruzi</i> II sylvatic isolates and TcII clones assessed using the gp 72 gene.

<p>The tree was constructed using the neighbor-joining method with Kimura-2-parameter distances. Bootstrap values are shown above major clades. MLD: <i>Leontopithecus rosalia</i> from Poço das Antas Biologic Reserve in the Atlantic Forest of the state of Rio de Janeiro: MLCD: <i>L. chrysomelas</i> from the Una Biologic Reserve in the Atlantic Forest of the state of Bahia, CD: dogs from the Cerrado in the state of Minas Gerais, LBT: <i>Rhodnius pictipes</i> from the Amazon Biome and isolate JCA3 is from a <i>Triatoma brasiliensis</i> from the state of Piauí in the Caatinga Biome. *GenBank sequences of strain TcI first published by Flores-López et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116137#pone.0116137-Briones1" target="_blank">[9]</a>.</p

<i>Trypanosoma cruzi</i> genotyping from naturally infected dogs and triatomines from the Amazon Biome.

<p>(A) PCR product size polymorphisms of the non-transcribed intergenic region of the SL-RNA mini-exon (mini-exon assay) of <i>T. cruzi</i> DNA from dog sera from Monte Alegre in the state of Pará, samples: 1- dog LBT 1818, 2- dog LBT 1819, 3- dog LBT 1820, 4- dog LBT 1821, and 5- dog LBT 1822 (B) Mini-exon assay of <i>Rhodnius pictipes</i> isolates from Abaetetuba (LBT 1458) and Belém (LBT 1814) in the state of Pará, Samples: 1- LBT 1458 and 2- LBT 1814 (C) <i>T. cruzi</i> genotyping profiles for PCR-RFLP with the 1f8 gene/Alw21I restriction enzyme of LBT 1458 clones 5 and 7, Samples: 1- LBT 1458 clone 5 and 2- LBT 1458 clone 7 (D) <i>T. cruzi</i> genotyping profiles for PCR-RFLP with histone 3/AluI restriction enzyme for the LBT 1814 isolate: Sample 1- LBT 1814 isolate. The <i>T. cruzi</i> DTUs, <i>T. rangeli</i> (H-14) and negative controls are indicated in the figure. DTU reference strains: I - Sylvio X/10 cl 1; II – Esmeraldo cl3; III – M5631 cl5; IV–92122102R; V – SC43 cl1; and VI – CL Brener. Agarose gel 3%, stained with ethidium bromide.</p

<i>Trypanosoma cruzi</i> molecular characterization in naturally infected hosts from the state of Pará, Brazil.

<p>(-) Not available;</p>a<p><i>Trypanosoma cruzi</i> kDNA positive serum samples from Xavier et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116137#pone.0116137-Xavier2" target="_blank">[27]</a>;</p>b<p>Mini-exon assay according to Fernandes et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116137#pone.0116137-Fernandes1" target="_blank">[29]</a>;</p>c<p>PCR-RFLP assay of the 1f8 gene according to Rozas et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116137#pone.0116137-Rozas1" target="_blank">[31]</a>;</p>d<p>PCR-RFLP assay of the histone 3 gene according to Westenberger et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116137#pone.0116137-Westenberger1" target="_blank">[32]</a>;</p>e<p>Sequence of a 250 bp fragment from the mini-exon gene. GenBank accession number KJ402456.</p><p><i>Trypanosoma cruzi</i> molecular characterization in naturally infected hosts from the state of Pará, Brazil.</p

<i>Trypanosoma cruzi</i> II isolates subjected to gp72 gene sequencing.

a<p>RJ - Rio de Janeiro; BA - Bahia; PI - Piauí; PA - Pará; MG - Minas Gerais;</p>b<p>A - according to profile described by Rozas et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116137#pone.0116137-Rozas1" target="_blank">[31]</a>; B - profile that was distinct form that described by Rozas et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116137#pone.0116137-Rozas1" target="_blank">[31]</a>.</p><p><i>Trypanosoma cruzi</i> II isolates subjected to gp72 gene sequencing.</p

Frequency of HTLV-1 infection in mother-offspring and spouse-spouse pairs in the endemic area of Argentina.

<p>Frequency of HTLV-1 infection in mother-offspring and spouse-spouse pairs in the endemic area of Argentina.</p

Common familiar polymorphisms on 552 nucleotides of LTR sequence from family isolates of Jujuy, considering J02029 as reference strain.

<p>Common familiar polymorphisms on 552 nucleotides of LTR sequence from family isolates of Jujuy, considering J02029 as reference strain.</p

Variables associated with HTLV-1 infection among families in the endemic area of Argentina.

<p>Variables associated with HTLV-1 infection among families in the endemic area of Argentina.</p

Family tree of index cases infected with HTLV-1.

<p>Family tree of index cases infected with HTLV-1.</p