Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans-9

<p><b>Copyright information:</b></p><p>Taken from "Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans"</p><p>Environmental Health Perspectives 2005;113(8):969-977.</p><p>Published online 18 May 2005</p><p>PMCID:PMC1280335.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans-8

<p><b>Copyright information:</b></p><p>Taken from "Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans"</p><p>Environmental Health Perspectives 2005;113(8):969-977.</p><p>Published online 18 May 2005</p><p>PMCID:PMC1280335.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

Hypothetical model of the molecular pathway involved in E-induced Ca regulation in α-cells

<p><b>Copyright information:</b></p><p>Taken from "Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans"</p><p>Environmental Health Perspectives 2005;113(8):969-977.</p><p>Published online 18 May 2005</p><p>PMCID:PMC1280335.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p> Abbreviations: G, G-protein; P, intra-cellular phosphate. 17β-E and EDCs activate a PTX-sensitive pathway, indicating either that a G-protein–coupled receptor (GPCR) is involved or that a receptor with a different structure from GPCRs is coupled to a classical GPCR that activates a G-protein. This receptor is coupled in a yet undetermined manner with NOS, which generates NO. This activates soluble GC, which increases cGMP levels, which activates PKG, regulating ion channels and producing the abolishment of low-glucose–induced [Ca]

Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans-5

<p><b>Copyright information:</b></p><p>Taken from "Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans"</p><p>Environmental Health Perspectives 2005;113(8):969-977.</p><p>Published online 18 May 2005</p><p>PMCID:PMC1280335.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans-7

<p><b>Copyright information:</b></p><p>Taken from "Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans"</p><p>Environmental Health Perspectives 2005;113(8):969-977.</p><p>Published online 18 May 2005</p><p>PMCID:PMC1280335.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans-3

<p><b>Copyright information:</b></p><p>Taken from "Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans"</p><p>Environmental Health Perspectives 2005;113(8):969-977.</p><p>Published online 18 May 2005</p><p>PMCID:PMC1280335.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans-1

<p><b>Copyright information:</b></p><p>Taken from "Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans"</p><p>Environmental Health Perspectives 2005;113(8):969-977.</p><p>Published online 18 May 2005</p><p>PMCID:PMC1280335.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans-6

<p><b>Copyright information:</b></p><p>Taken from "Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca Signals in Pancreatic α-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans"</p><p>Environmental Health Perspectives 2005;113(8):969-977.</p><p>Published online 18 May 2005</p><p>PMCID:PMC1280335.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

STX does not change glucose homeostasis in ovariectomized female mice.

<p>A, Intraperitoneal glucose tolerance test (IPGTT) in ovariectomized female mice after one single dose of vehicle (•) or 100 µg/kg STX (○). B, Area under the curve in the IPGTT in A; n = 7–8 mice/condition. C, Body weight in ovariectomized female mice before and after the 6 day-treatment with either vehicle or STX; n = 5–6 mice/condition. D, Fasting glycemia in the following morning after the 6-day treatment with vehicle or STX treated mice (n = 5–6 mice/condition). E, IPGTT in the same mice as in D; vehicle (•), STX (○); n = 5–6 mice/condition. F, Insulin content in isolated islets from vehicle and STX-treated ovariectomized female mice; n = 110–140 islets from 4–6 mice/condition.</p

STX does not alter insulin content or blood glucose homeostasis at long-term in male mice.

<p>A, Body weight in male mice before and after the 6 day-treatment with either vehicle or STX; n = 5 mice/condition. B, Fasting glycemia in the following morning after the 6-day treatment with vehicle or STX treated mice (n = 5 mice/condition). C, IPGTT in the same mice as in B; vehicle (•), STX (○); n = 4–5 mice/condition. D, Insulin content in isolated islets from vehicle and STX-treated mice; n = 60–120 islets from 2–3 mice/condition.</p