STUDY OF OUTWARD SIGNS, MICROSCOPY AND CHEMICAL COMPOSITION OF WALNUT

Objective: Walnut is a medicinal plant that is widely used in traditional medicine. We believe that the study of the medicinal plant material of walnut-fruit in the stage of milky-wax maturity and walnut bark is relevant. We described outward signs and microscopy, the diagnostic features of both whole and grinded raw materials, powder bark of walnut and fruits of walnut in the stage of milky-wax maturity.Methods: Alcohol extraction which was derived from walnut bark is a transparent liquid of brown color and alcohol extraction which was derived walnut fruits in the stage of milky-waxy maturity is a transparent, green-brown liquid with a fragrant smell. We performed thin-layer chromatography for the walnut cortex and identified gallic acid.Results: A method of chromatography-mass spectrometry in alcohol extraction from walnut fruit in the stage of milky-waxy maturity allowed to identify 17 compounds belonging to different classes of biologically active substances. We identified sugars, flavonoids, phenolic compounds of coumarins and organic acids. In alcohol extraction from the walnut bark we identified marker substances

LIQUID CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS OF FRUITS OF AN ECONOMICALLY IMPORTANT PLANT SCHISANDRA CHINENSIS BAIL. FROM CHINA AND EAST RUSSIA

Objective: The study was carried out with an objective to characterize the possible bioactive phytochemical constituents from fruits of Schisandra chinensis Bail. by liquid chromatography–mass spectrometry analysis.Methods: Plant material was collected from Schisandra chinensis during August–October. The dried plant fruits were extracted with solvents using ethanol 95% extractor. The results of chromatography–MS analysis performed on the instrument Agilent Technologies established the presence of major and minor components. It was conducted a qualitative and quantitative comparison of infusions using software ChemStationE 02.00 and full library of mass spectra NIST 05