1 research outputs found
Multiplexed Imaging of Nanoparticles in Tissues Using Laser Desorption/Ionization Mass Spectrometry
Imaging
of nanomaterials in biological tissues provides vital information
for the development of nanotherapeutics and diagnostics. Multiplexed
imaging of different nanoparticles (NPs) greatly reduces costs, the
need to use multiple animals, and increases the biodistribution information
that can enhance diagnostic applications and accelerate the screening
of potential therapeutics. Various approaches have been developed
for imaging NPs; however, the readout of existing imaging techniques
relies on specific properties of the core material or surface ligands,
and these techniques are limited because of the relatively small number
of NPs that can be simultaneously measured in a single experiment.
Here, we demonstrate the use of laser desorption/ionization mass spectrometry
(LDI-MS) in an imaging format to investigate surface chemistry dictated
intraorgan distribution of NPs. This new LDI-MS imaging method enables
multiplexed imaging of NPs with potentially unlimited readouts and
without additional labeling of the NPs. It provides the capability
to detect and image attomole levels of NPs with almost no interferences
from biomolecules. Using this new imaging approach, we find that the
intraorgan distributions of same-sized NPs are directly linked to
their surface chemistry