Two <i>Canna</i> cultivars having contrasting flower color (A) Tropical sunrise (B) Red president.

<p>Two <i>Canna</i> cultivars having contrasting flower color (A) Tropical sunrise (B) Red president.</p

Gene ontology enrichment analysis of the predicted targets of <i>Canna</i> miRNAs.

<p>Categorization of miRNA-targets genes were performed according to the(A) Biological Processes, (B) Molecular function and (C) Cellular component.</p

Differentially expressed miRNA families between TS and RP.

<p>Differentially expressed miRNA families between TS and RP.</p

Comparison of the miRNA expression levels determined by deep sequencing and qRT-PCR.

<p>Blue and red colors indicate the fold change obtained by deep sequencing and qRT-PCR, respectively. The error bars indicate the standard deviation obtained from biological and technical replicates.</p

The homology of identified miRNAs with other plant species.

<p>Values on Y axis indicate the number of homological miRNA families between <i>Canna</i> and other plant species.</p

Flavonoids, Carotenoids, Xanthophyll and Anthocyanin content of TS and RP.

<p>All the measurements were taken in three biological and five technical replicates. Error bars represent standard deviation of biological and technical replicates.</p

Venn diagrams of conserved and unique miRNAs between TS and RP.

<p>(A) Total conserved and unique miRNAs between TS and RP, (B) Conserved and unique miRNA families between TS and RP, (C) Total conserved and unique miRNA* between TS and RP, (D) Conserved and unique miRNA* families between TS and RP.</p

Detection of cleavage site through RLM-RACE.

<p>5' RLM RACE was used to map the cleavage sites. The partial mRNA sequence from the target genes were aligned with the miRNA. The arrow indicates the cleavage site, and the number above the arrow denotes the frequency of the sequenced clones.</p

Number of miRNAs members in each family identified from TS and RP by deep sequencing.

<p>Number of miRNAs members in each family identified from TS and RP by deep sequencing.</p