1 research outputs found
Antioxidant, anti-inflammatory and anti-arthritic activities of methanol extract of Tamus communis L. roots
Purpose: To evaluate the antioxidant, anti-inflammatory and antiāarthritic effects of the methanol extract of Tamus communis roots.
Methods: The total phenolic and flavonoid contents were determined using FolināCiocalteuās reagent and aluminium chloride assays, respectively while the antioxidant capacity was determined using DPPH and Ī²-carotene/linoleate tests. For anti-inflammatory activity, carrageenan-induced paw edema in rat was used. Formaldehyde-induced paw edema and adjuvant-induced arthritis in rats were used to evaluate the anti-arthritic effect of the extract.
Results: The total phenolic and flavonoid contents of T. communis showed 55.2 Ā± 0.036 mg GAE/g dry weight and 1.191 Ā± 0.002 mg QEq/g dry weight, respectively while the antioxidant activity (DPPH) showed a half-maximal inhibitory concentration (IC50 of 0.128 Ā± 0.011 mg/mL. Using Ī²carotene/linoleate assay, the extract showed 88.13 Ā± 4.03 % inhibition. In carrageenan-induced paw edema in rat, all doses of T. communis did not show inhibition of edema. In contrast, formaldehyde induced paw edema decreased at the dose of 150 mg/kg days 2 and 4. However, doses of 300 and 600 mg/kg did not show any activity. In adjuvant-induced arthritis, all used doses caused no reduction in arthritic scores and in paw thickness except a significant decrease with the dose 150 mg/kg at the last day.
Conclusion: T. communis extract exhibits high antioxidant activity related to the phenolic compound contents. However, the results of the anti-inflammatory and antiāarthritic studies did not support its use in folk medicine in the treatment of rheumatic ailment except on low dose