2 research outputs found
Reactivity and Biological Properties of a Series of Cytotoxic PtI<sub>2</sub>(amine)<sub>2</sub> Complexes, Either <i>cis</i> or <i>trans</i> Configured
Six diiodidoādiamine platinumĀ(II) complexes, either <i>cis</i> or <i>trans</i> configured, were prepared,
differing only in the nature of the amine ligand (isopropylamine,
dimethylamine, or methylamine), and their antiproliferative properties
were evaluated against a panel of human tumor cell lines. Both series
of complexes manifested pronounced cytotoxic effects, with the <i>trans</i> isomers being, generally, more effective than their <i>cis</i> counterparts. Cell cycle analysis revealed different
modes of action for these new PtĀ(II) complexes with respect to cisplatin.
The reactivity of these platinum compounds with a number of biomolecules,
including cytochrome c, two sulfur containing modified amino acids,
9-ethylguanine, and a single strand oligonucleotide, was analyzed
in depth by mass spectrometry and NMR spectroscopy. Interestingly,
significant differences in the reactivity of the investigated compounds
toward the various model biomolecules were observed: in particular
we observed that <i>trans</i> complexes preferentially release
their iodide ligands upon biomolecule binding, while the <i>cis</i> isomers may release the amine ligands with retention of iodides.
Such differences in reactivity may have important mechanistic implications
and a relevant impact on the respective pharmacological profiles
Reactivity and Biological Properties of a Series of Cytotoxic PtI<sub>2</sub>(amine)<sub>2</sub> Complexes, Either <i>cis</i> or <i>trans</i> Configured
Six diiodidoādiamine platinumĀ(II) complexes, either <i>cis</i> or <i>trans</i> configured, were prepared,
differing only in the nature of the amine ligand (isopropylamine,
dimethylamine, or methylamine), and their antiproliferative properties
were evaluated against a panel of human tumor cell lines. Both series
of complexes manifested pronounced cytotoxic effects, with the <i>trans</i> isomers being, generally, more effective than their <i>cis</i> counterparts. Cell cycle analysis revealed different
modes of action for these new PtĀ(II) complexes with respect to cisplatin.
The reactivity of these platinum compounds with a number of biomolecules,
including cytochrome c, two sulfur containing modified amino acids,
9-ethylguanine, and a single strand oligonucleotide, was analyzed
in depth by mass spectrometry and NMR spectroscopy. Interestingly,
significant differences in the reactivity of the investigated compounds
toward the various model biomolecules were observed: in particular
we observed that <i>trans</i> complexes preferentially release
their iodide ligands upon biomolecule binding, while the <i>cis</i> isomers may release the amine ligands with retention of iodides.
Such differences in reactivity may have important mechanistic implications
and a relevant impact on the respective pharmacological profiles