Predicted targets of <i>miR-142-3p</i> and <i>miR-142-5p</i> using Diana-MicroT and Target Scan software prediction tools.

<p><b>(A)</b> Pathway intersection between <i>miR-142-3p</i> and <i>miR-142-5p</i>. <b>(B)</b> Predicted targets of <i>miR-142-3p</i> and <i>miR-142-5p</i> in the Wnt signaling pathway. Orange boxes: common predicted targets for both isoforms; yellow boxes: predicted targets for both isoforms; green boxes: experimentally validated target of <i>miR-142-3p</i>; red box: experimentally validated target of <i>miR-142-5p</i>.</p

<i>Increased expression of Apc and p300 is associated with increased Wnt signaling in E18</i>.<i>5 miR-142</i>-null <i>lungs</i>.

<p>(<b>A-L)</b> Immunoflorescence for activated beta-catenin <b>(A-D)</b>, Adenomatous polyposis coli (Apc) <b>(E-H)</b> and p300 <b>(I-L)</b> in wild type (A,B, E,F,I,J) and <i>miR-142</i> ko (C,D,G,H,K,L) lungs showing increased Wnt signaling, Apc and p300 expression. <b>(M-N)</b> qPCR analysis of beta catenin (<i>Ctnnb1</i>), <i>Apc</i> and <i>p300</i> expression in E18.5 wild type and <i>miR-142</i>-null lungs. Scale bar: (A,C,E,G,I,K): 44 μm and (B,D,F,H,J,L): 9 μm.</p

<i>In situ</i> hybridization on E18.5 <i>miR-142</i>^<i>-/-</i> embryonic lung sections.

<p>In situ hybridization for <i>miR-142-3p</i> (<b>A-D</b>) and <i>miR-142-5p</i> (<b>E-H</b>) showing significant reduction of the corresponding expression levels in <i>miR-142</i>^<i>-/-</i> compared to WT E18.5 embryonic lung sections. (<b>I-J</b>). Quantification of the in situ hybridization signals indicating a strong reduction of both isoforms. Scale bar: (A,C,E,G): 20 μm and (B,D,F,H): 4 μm.</p

<i>miR-142</i> KO mice display hematological abnormalities.

<p><b>(A</b>) Schematic representation of hematopoietic stem cells differentiated into different blood cell types. Peripheral blood cell count in 8 week old <i>miR-142</i>^<i>-/-</i> mice (n = 3) showing a significant decrease in circulating white blood cells (<b>B</b>), lymphocytes <b>(C</b>), platelets (<b>D</b>), eosinophils (<b>E</b>) and monocytes (<b>G</b>) in addition to an increase in mean platelet volume (<b>F</b>).</p

Number of embryos obtained at different stages of embryonic development.

<p># 3 animals died at the age of 2–3 months</p><p>* 1 animal died at the age of 4 months</p><p>Number of embryos obtained at different stages of embryonic development.</p