Histological changes in the endometrial of pregnant Sprague-Dawley rats under supplementation levels of n-6:n-3 fatty acid ratio

This study describes a changed uterine morphometry and its application to the endometrial structure of a pregnant rat. The number and the size of uterine gland and blood vessels changed during the pregnancy period of the rat. This effect on day 15 was significantly changed in the different groups. When the endometrial morphology was related to the circulating progesterone concentrations on day 15, it was observed that relationships were found on day 15 and a high progesterone concentration in the Diet 1:1 group was associated with an increased number of the uterine gland and blood vessel. Furthermore, animals in the Diet 1:30 group were slaughtered on day 15 and a low progesterone concentration on that same day was associated with the decreased uterine gland size, though a simultaneous decrease was found in the number of endometrial gland. In contrast, the number of implanted embryos was significantly lower in the Diet 3 group at 15 days of gestation The results suggest that the endometrial glands do not only grow and regress during the supplementation of high ratio n-6:n-3 fatty acids, but the number and size of the endometrial glands in the endometrial area are controlled by progesterone, which leads to changes in the structure and maintenance of the uterine during the pregnancy period.Key words: n-6:n-3 Fatty acid ratio, progesterone, blood vessel, uterine gland, pregnant rat

Effects of varying levels of n-6:n-3 fatty acid ratio on plasma fatty acid composition and prostanoid synthesis in pregnant rats

This study investigated the effects of varying dietary levels of n-6:n-3 fatty acid ratio on plasma fatty acid composition and prostanoid synthesis in pregnant rats. Four groups consisting of seven rats pergroup of non pregnant rats were fed diets with either a very low n-6:n-3 ratio of 50% soybean oil (SBO): 50% cod liver oil (CLO) 1:1 group , a low ratio of 84% SBO: 16% CLO 6:1 group, a high ratio of 96% SBO: 4% CLO 30:1 group and control group was given only rat chow diet. Blood samples were taken at day 15 post mating and the plasma was analyzed for fatty acid profile, specifically the n-6:n-3 fatty acid ratio and prostaglandins F2α and E2. The n-3 polyunsaturated fatty acids (PUFA) in plasma of group 1:1 were significantly (P < 0.05) higher than the other groups, while the n-6:n-3 fatty acid ratio was significantly lower. The total n-6 PUFA was significantly higher (P < 0.05) in group 30:1 as compared to the control and 1:1 groups. The total PGF2α and PGE2 were significantly higher (P < 0.05) in group 30:1 rats fed a diet high in n-6 or n-6:n-3 fatty acids. The diet higher in n-6 fatty acids appear to increase arachidonic acid( AA) and prostaglandins synthesis in plasma of rats. PGE2 productions in plasma were significantly lower in rats fed diets with a lower dietary ratio of n-6:n-3 fatty acids than in those fed diets with a higher dietary ratio. Regression analysis revealed a significant positivecorrelation between PGF2α and PGE2 and the ratio of n-6:n-3, and significant positive correlation between different ratio n-6:n-3 on fatty acid plasma compstion and PGF2α and PGE2 concentration on plasma. These results demonstrated that the dietary ratio of n-6:n-3 modulates PGF2α and PGE2 production. The n-6:n-3 fatty acid ratio significantly affected plasma fatty acids profile and prostaglandin synthesis in pregnant rat.Keywords: n-6:n-3 fatty acid ratio, plasma fatty acids, prostanoid synthesis, pregnant ra