Tumorigenicity in old A53T α-Syn +/+ and control mice.

<p>(a). Mice (9–10 months old) were injected with B16 melanoma cells and tumor volumes were determined as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0019622#pone-0019622-g001" target="_blank">Fig. 1a</a>. The experiment was stopped after 20 days, when the tumors in any genotype group reached 1 to 1.2 cm in diameter. The graph represents the means ± standard errors (SE) of 8–9 mice in each group. (b). Mice were injected with D122 Lewis lung carcinoma cells and tumor volume was measured as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0019622#pone-0019622-g001" target="_blank">Fig. 1a</a>. Mean weights of tumors formed in each group at 20 days post- injection ± SE for B16 (c); and D122 (d). *, p<0.05 Mann-Whitney test.</p

Tumorigenicity of B16 melanoma is not affected in APP/PS1 tg mice.

<p>(a). Mice (3–4 months old) were injected subcutaneously with 2.5×10⁵ B16 melanoma cells and tumor volumes were determined every 1–2 days post injection. The experiment was stopped after 14 days, when the tumors in the A53T α-Syn+/+ group reached 1 to 1.2 cm in diameter. The graph represents the means ± standard errors (SE) of 5–7 mice in each group. (b). Mean weights of tumors formed in each group at 14 days post- injection ± SE. *, p<0.05, Mann-Whitney test.</p

α-Syn expression in B16 and E0771 enhances cell proliferation.

<p>(a). Stable poly-clones of B16 cells over expressing either human wt α-Syn, human β-Syn, amyloid precursor protein carrying the Swedish mutation (APPsw) or mock-transfected, were seeded in a 96-well plates at 5×10³ cells per well. Proliferation was determined by the fluorescence ratio at 560ex/590em and normalized to the mock-transfected cells. A representative result of cells 48 hours post seeding. Mean ± SE of n = 6 wells out of three repeats. (b) Stable poly-clones of E0771 and (c) D122, seeded and measured as in (a). *, p<0.05, Mann-Whitney test.</p

Tumorigenicity in young A53T α-Syn +/+ and control F1 B6/C3H mice.

<p>(a). Mice (3–4 months old) were subcutaneously injected with 0.25×10⁶ B16 melanoma cells. Tumor volumes were determined every 1–2 days post injection. The experiment was stopped after 15 days, when the tumors in the A53T α-Syn +/+ group reached 1 to 1.2 cm in diameter. The graph represents the means ± standard errors (SE) of 5–8 mice in each group. A representative growth curve out of three independent experiments. (b). Mice were injected with 1×10⁵ E0771 mammary gland adenocarcinoma cells and tumor volume was measured as in (a). (c). Mice were injected with 5×10⁴ D122 Lewis lung carcinoma and tumor volume was measured as in (a). (d). Mean weights of tumors formed in each genotype group at 15 days post- injection ± SE for B16, *,p = 0.015, t-test; (e). E0771, *, p = 0.012, t-test; (f). D122; (g). Mean weights of tumors formed in each genotype group at 15 days post- injection ± SE, n = 6–7, *, p<0.05 Mann-Whitney test.</p