DNA yield and purity of isolated DNA from the eight cannabis resin samples using Wagner and adapted CTAB protocol (all DNA were re-dissolve in 100 µl sterile deionised water).

<p>DNA yield and purity of isolated DNA from the eight cannabis resin samples using Wagner and adapted CTAB protocol (all DNA were re-dissolve in 100 µl sterile deionised water).</p

(A): Agarose gel analysis (0.8%) of extracted gDNA by the adapted protocol. (B) : Agarose gel analysis (0.8%) of extracted gDNA by the Wagner protocol. (1–8): Indicated number of samples; (L): 10 kb molecular size ladder with the position of 500 bp, 1000 bp and 10 kb fragments indicated.

<p>(A): Agarose gel analysis (0.8%) of extracted gDNA by the adapted protocol. (B) : Agarose gel analysis (0.8%) of extracted gDNA by the Wagner protocol. (1–8): Indicated number of samples; (L): 10 kb molecular size ladder with the position of 500 bp, 1000 bp and 10 kb fragments indicated.</p

Sequences of oligonucleotides used in this study.

<p>Sequences of oligonucleotides used in this study.</p

Example of compressed cannabis resin (Hashish) confiscated by Moroccan customs.

<p>Left hand: heated block of Hashish. Right hand: unheated block of Hashish.</p

Agarose gel electrophoresis (1%) of PCR amplification product of THCA synthase gene using the primers (a/b).

<p>(A): Amplification of DNA extracted by the adapted protocol. (B1): Amplification of DNA extracted by Wagner CTAB protocol (cannabis resin). (1–8): Indicated number of samples; (L): 100 bp molecular size ladder; the position of 100 bp, 600 bp and 1600 bp fragments is indicated. (B2): Amplification of DNA extracted by Wagner CTAB protocol (using other tissues of <i>cannabis sativa</i>). (GL): Grind leaves of cannabis sativa. (S): Seeds of cannabis. (R): Rod of cannabis sativa. (M): Mixed rood and leaves. (T): Negative control.</p