1 research outputs found
Binding mechanism of methyl-α-N-acetyl-D-galactopyranosyl amine to <i>Artocarpus lakoocha </i>lectin, artocarpin: A proton nuclear magnetic resonance study
299-306The dynamics of the binding mechanism
between Artocarpus lakoocha lectin and Me-α-D-GalNAc has been studied using
1H NMR spectroscopy. Various thermodynamic parameters have been calculated
with the help of temperature dependence of line broadening of the methoxy group
resonance of Me-α-D-GalNAc. No change in the chemical shift has
been observed while full line width at half
height of the sugar protons was found to increase with increasing temperature indicating
that the binding ligand is in fast exchange. No chemical shift between bound and
free ligands has been observed.The activation parameters obtained from the association
and dissociation rate constants suggest that the association process is controlled
by high activation entropy which is due to the specific orientation of both lectin
and sugar whereas the contribution of activation enthalpy is small. On the other
hand, the dissociation reaction is controlled by high activation enthalpy due
to the break in the interaction between the sugar and the lectin. From NMR data
a two-step binding mechanism has been proposed. The associated complex is stabilized
mainly by hydrogen bonding and vander Waals attractions while hydrophobic
interaction is not significant as indicated by the negative entropy and enthalpy
values