Antibacterial and antioxidant constituents of <i>Acalypha wilkesiana</i>

<div><p>This study was aimed at characterising the secondary metabolites responsible for antibacterial and antioxidant activities of <i>Acalypha wilkesiana</i>. Purification of the defatted methanol leaves extract was guided by the DPPH free radical scavenging assay as well as by evaluation of the antibacterial activity against four bacterial strains. As a result, geraniin, corilagin, quadrangularic acid M and shikimic acid were purified and isolated. Shikimic acid, reported for the first time from this plant, proved to be the major metabolite of the extract. All the four isolated compounds showed bactericidal activity against extended spectrum beta-lactamase-producing <i>Klebsiella pneumoniae</i> (700603), while corilagin was the single compound to exhibit antioxidant activity (IC₅₀ 53 μg/mL).</p></div

Evaluation of a Silver-Embedded Ceramic Tablet as a Primary and Secondary Point-of-Use Water Purification Technology in Limpopo Province, S. Africa

<div><p>The World Health Organization (WHO) recognizes point-of-use water treatment (PoUWT) technologies as effective means to improve water quality. This paper investigates long-term performance and social acceptance of a novel PoUWT technology, a silver-infused ceramic tablet, in Limpopo Province, South Africa. When placed in a water storage container, the silver-embedded ceramic tablet releases silver ions into water, thereby disinfecting microbial pathogens and leaving the water safe for human consumption. As a result of its simplicity and efficiency, the silver-embedded ceramic tablet can serve as a stand-alone PoUWT method and as a secondary PoUWT to improve exisitng PoUWT methods, such as ceramic water filters. In this paper, three PoUWT interventions were conducted to evaluate the silver-embedded ceramic tablet: (1) the silver-embedded ceramic tablet as a stand-alone PoUWT method, (2) ceramic water filters stand-alone, and (3) a filter-tablet combination. The filter-tablet combination evaluates the silver-embedded ceramic tablet as a secondary PoUWT method when placed in the lower reservoir of the ceramic water filter system to provide residual disinfection post-filtration. Samples were collected from 79 households over one year and analyzed for turbidity, total silver levels and coliform bacteria. Results show that the silver-embedded ceramic tablet effectively reduced total coliform bacteria (TC) and <i>E</i>. <i>coli</i> when used as a stand-alone PoUWT method and when used in combination with ceramic water filters. The silver-embedded ceramic tablet’s performance as a stand-alone PoUWT method was comparable to current inexpensive, single-use PoUWT methods, demonstrating 100% and 75% median reduction in <i>E</i>. <i>coli</i> and TC, respectively, after two months of use. Overall, the the filter-tablet combination performed the best of the three interventions, providing a 100% average percent reduction in <i>E</i>. <i>coli</i> over one year. User surveys were also conducted and indicated that the silver-embedded ceramic tablet was simple to use and culturally appropriate. Also, silver levels in all treated water samples remained below 20 μg/L, significantly lower than the drinking water standard of 100 μg/L, making it safe for consumption. Long-term data demonstrates that the silver-embedded ceramic tablet has beneficial effects even after one year of use. This study demonstrates that the silver-embedded ceramic tablet can effectively improve water quality when used alone, or with ceramic water filters, to reduce rates of recontamination. Therefore, the tablet has the potential to provide a low-cost means to purify water in resource-limited settings.</p></div

Average percent reduction of total coliform over time.

<p>Samples were treated with silver-embedded ceramic tablet (SCT), ceramic water purification systems (CWF) or ceramic water purification systems with the silver-embedded ceramic tablet (CWF+SCT). Percent reduction was calculated based off of control ceramic tablets (for SCT-treated samples) or source water (for CWF- and CWF+SCT-treated samples). Data points represent average and error bars represent standard error.</p

Total coliform bacteria in water storage containers with ceramic tablets.

<p>Control samples represent samples taken from the water storage container with the control ceramic tablet. Silver ceramic tablet samples represent samples taken from water storage containers with the silver-embedded ceramic tablet. Data points represent average total coliform levels among all households per week. Standard error was used to calculate error bars.</p

Correlation of turbidity and percent reduction in bacteria among households using the SCT method.

<p>Samples were treated with silver-embedded ceramic tablets and analyzed for reduction in total coliform bacteria (A) and E. coli (B) turbidity. Samples were taken at 37 and 52 weeks post-intervention.</p

WHO risk category of samples from households using SCT, CWF and CWF+SCT POU water treatment methods.

<p>WHO risk category of samples from households using SCT, CWF and CWF+SCT POU water treatment methods.</p

Total silver levels among water samples taken from households using ceramic water purification systems over 12 months.

<p>(A) Silver concentrations among households were either using the ceramic water purification system (CWF) or with the silver-embedded ceramic tablet (CWF+SCT). (B) Total silver concentrations among households with water storage containers with control and silver-embedded ceramic tablets. Data points represent average silver concentrations. Standard error was used to calculate error bars.</p

Incidence of EAEC.

<p>Cumulative incidence of first EAEC detection in A) surveillance and diarrheal stools at all sites and B) surveillance stools by site among 2,092 children with at least one stool sample in the MAL-ED birth cohort. BGD–Dhaka, Bangladesh; BRF–Fortaleza, Brazil; INV–Vellore, India; NEB–Bhaktapur, Nepal; PEL–Loreto, Peru; PKN–Naushahro Feroze, Pakistan; SAV–Venda, South Africa; TZH–Haydom, Tanzania.</p

Associations between EAEC detection and markers of inflammation and gut permeability in surveillance and diarrheal stools among 2,076 children in the MAL-ED cohort with at least one biomarker measurement.

<p>Associations between EAEC detection and markers of inflammation and gut permeability in surveillance and diarrheal stools among 2,076 children in the MAL-ED cohort with at least one biomarker measurement.</p

Effects of EAEC detection in monthly surveillance stools on weight (WAZ) and length (LAZ) attainment at 2 years of age among 1,727 children in the MAL-ED cohort with anthropometric measurements at 2 years.

<p>Effects of EAEC detection in monthly surveillance stools on weight (WAZ) and length (LAZ) attainment at 2 years of age among 1,727 children in the MAL-ED cohort with anthropometric measurements at 2 years.</p