Snorkel Research on Anadromous Fishes (Poster)

Dam construction has had a large impact on anadromous fish in the Pacific Northwest. All anadromous salmonids in the Pacific Northwest have been deemed as endangered species.  In addition, climate change, and commercial and sport fishing have also had an impact on the anadromous populations. As a result, state and federal organizations have made an effort to augment populations with hatcheries, habitat restoration, and improved fish passages through dam systems. In efforts to understand the tributaries and spawning grounds used by chinook salmon and steelhead trout, the Idaho Department of Fish and Game has created population research groups in the form of snorkel crews. One goal of the snorkel crew was to gain a better understanding of which tributaries are frequented by each species of fish. Snorkel transects consisted of regular sites that were snorkeled annually, as well as sites chosen at random.  Transects were lengths of water from 65 to 200 meters long. Snorkelers moved in a serpentine pattern through the stream to cover maximal area. Fish size, number and species were recorded only after fish were passed by a snorkeler. Periodically, the snorkeler would relay data to a person nearby designated to data collection. Sites were chosen at random to be evaluated using a mark re-sight method to estimate efficiency. Anglers fished an area, caught fish were marked and recorded and then released. After fishing the site was left untouched for at least 24 hours before it was snorkeled. After snorkeling an in depth habitat evaluation was conducted

Technique [Volume 87, Issue 25]

2002 Housing Guide: Decisions, decisions, decisions-to live on campus, or off?2002 Housing Guide: The off-campus housing cheat sheetAustralia's Kasey Chambers makes bluegrass hip with latest albumBeyond the White and GoldCorrectionCouncil Clippings: HouseCouncil Clippings: SenateDurham travels from roller rink to gridironGiddens named new COE DeanIntramural NotebookLady Jackets look for post-season bidLetters to the EditorMen's swimming out to prove season is no flukeMorisette's 'Under Rug Swept' not to be discardedNew initiative set to 'integrate' TechNews BriefsOUR VIEWS: Consensus OpinionSports BriefsSTAFF EDITORIAL:Tech (14-15, 6-9 ACC) at FSU (11-15, 4-11 ACC), 12 p.m. on FSNSTech and the Legislature: Budget cuts may affect InstituteTechs and the City:The Live List:...Two Bit

Technique [Volume 87, Issue 11]

ACM debate forces UHR roll call voteACM debate forces UHR roll call voteBeyond the White and GoldCompetitive ExistenceCOT gains over twenty new women in first rushDantzler provides Twilight Zone moments against uninspired defenseDo you know your rights? Understand sexual harassmentFlying club back in air after attacksGSS supports bill to endow Team BuzzHawke, Washington ready for 'Training Day'How big a 'change' is changing majors?Incubus rocks TabernacleInstitute finalizes Greek master planLCC exodus: Eight professors leave School, more rumoredMicrogravity study takes Jackets to new heightsMoving on: Tech puts Clemson in pastNews BriefsOffice Supplies-Harmless Fetish or Dangerous Liaison?OUR VIEWS Consensus OpinionSaving football's crown jewel from players and...oranges?STAFF EDITORIAL: Grads should not fear reaching out to those in needSTAFF EDITORIAL: Students should get to know Tech campus buildingsTechs and the City:The Live List: 10/5 to 10/12...Two BitsUrban atmosphere apparent at Apach

Persistent STAT5 phosphorylation and epigenetic dysregulation of GM-CSF and PGS2/COX2 expression in Type 1 diabetic human monocytes.

STAT5 proteins are adaptor proteins for histone acetylation enzymes. Histone acetylation at promoter and enhancer chromosomal regions opens the chromatin and allows access of transcription enzymes to specific genes in rapid response cell signals, such as in inflammation. Histone acetylation-mediated gene regulation is involved in expression of 2 key inflammatory response genes: CSF2, encoding granulocyte-macrophage colony stimulating factor (GM-CSF), and PTGS2, encoding prostaglandin synthase 2/cyclooxygenase 2 (PGS2/COX2). Prolonged CSF2 expression, high GM-CSF production, and GM-CSF activation of PTGS2 gene expression all are seen in type 1 diabetes (T1D) monocytes. Persistent phosphorylation activation of monocyte STAT5 (STAT5Ptyr) is also found in individuals with or at-risk for T1D. To examine whether elevated T1D monocyte STAT5Ptyr may be associated with aberrant inflammatory gene expression in T1D, blood monocytes from non-autoimmune controls and T1D patients were analyzed by flow cytometry for STAT5Ptyr activation, and by chromatin immuno-precipitation (ChIP) analyses for STAT5Ptyr's ability to bind at CSF2 and PTGS2 regulatory sites in association with histone acetylation. In unstimulated monocytes, STAT5Ptyr was elevated in 59.65% of T1D, but only 2.44% of control subjects (p<0.0001). Increased STAT5Ptyr correlated with T1D disease duration (p = 0.0030, r(2) = 0.0784). Unstimulated (p = 0.140) and GM-CSF-stimulated (p = 0.0485) T1D monocytes, had greater STAT5Ptyr binding to epigenetic regulatory sites upstream of CSF2 than control monocytes. Increased STAT5Ptyr binding in T1D monocytes was concurrent with binding at these sites of STAT6Ptyr (p = 0.0283), CBP/P300 histone acetylase, acetylated histones H3, SMRT/NCoR histone deacetylase (p = 0.0040), and RNA Polymerase II (p = 0.0040). Our study indicates that in T1D monocytes, STAT5Ptyr activation is significantly higher and that STAT5Ptyr is found bound to CSF2 promoter and PTGS2 enhancer regions coincident with histone acetylation and RNA polymerase II. These findings suggest that the persistent activation of STAT5 by GM-CSF may be involved in altering the epigenetic regulation of these inflammatory response genes in T1D monocytes

Characteristics of Patients with Non-Cancer Pain and Perceived Severity of COVID-19 Related Stress

UNLABELLED: Synopsis Patients with non-cancer pain reported increased pain and pain interference during the first months of the COVID-19 pandemic. We determined if pain, prescription opioid use, and comorbidities were associated with perceived COVID-19-related stress as the pandemic peaked. Analysis of survey data revealed that depression/anxiety, pain severity, and pain interference were most strongly and consistently associated with greater stress due to COVID-19 related changes in lifestyle, worsening of emotional/mental health and worsening pain. Identifying specific stressful experiences that most impacted patients with non-cancer pain may help target public health and treatment interventions. BACKGROUND: During the first months of the COVID-19 pandemic, patients with chronic pain reported increased pain severity and interference. This study measured the association between pain, prescription opioid use, and comorbidities with perceived COVID-19-related stress as the pandemic peaked in the United States. METHODS: From 9/2020 to 3/2021, the first 149 subjects from a prospective cohort study of non-cancer pain, completed a survey which contained the Complementary and Integrative Research (CAIR) Pandemic Impact Questionnaire (C-PIQ). Respondents also reported whether the pandemic has contributed to their pain or opioid use. Bivariate comparisons explored patient characteristics with each CAIR domain. RESULTS: Respondents mean age was 54.6 (±11.3) years, 69.8% were female, 64.6% were White. Respondent characteristics were not associated with reading/watching/thinking about the pandemic or with worry about health. Depression/anxiety (p=0.003), using any prescription opioid in the prior three months (p=0.009), higher morphine milligram equivalent used (p=0.005), higher pain severity (p=0.011), and higher pain interference (p=0.0004) were all positively and significantly associated with moderate to severe stress due to COVID-19 related lifestyle changes. Depression/anxiety, pain severity, and pain interference were positively associated with COVID-19-related worsening emotional/mental health. Depression/anxiety were significantly (p CONCLUSION: Depression, anxiety, pain severity, and pain interference were most strongly and consistently associated with COVID-19 changes in way of life, worsening of emotional/mental health, and worsening pain. Identifying specific stressful experiences that most impacted patients with noncancer pain may inform public health and treatment interventions

Technique [Volume 87, Issue 30]

Admissions numbers show slight decreaseAn Untitled PerspectiveBaseball Midseason ReportBeyond the White and GoldCampus Event RecapClub crew team leaving big wakeCouncil Clippings: HouseCouncil Clippings: SenateEntertainment BriefsFaces at Georgia TechGay students spend day without speakingGreeks fight safety, weather problemsGreeks reject Dean's planIntramural NotebookLetters to the EditorNews BriefsNo. 1 Tech golf looks for repeat title at ACC tourneyOUR VIEWS Consensus OpinionProvost beefs up Dead Week policyQB Battle: Down to twoRecruiting the next class of JacketsRoth IRAs: So you want to have cash when you retire?Sliver BoxSneaker Pimps offer moody electronicaSports BriefsStaff Editorial:Techs and the City:The Live List: April 12th onwardThrilling Superman ride at Six Flags takes its riders to new heights...Two Bit

Technique [Volume 87, Issue 21]

Beyond the White and GoldCollege briefsComputing dean resigns to take job with NSFCooking in your dorm (or 'Beyond Ramen Noodles')Council Clippings: HouseCouncil Clippings: SenateFootball recruiting class shows Gailey's Texas connectionsForward Ed Nelson making big impactHow to eat lunch like a comparison shopperIntramural NotebookJosé Carreras wows sold-out Ferst crowdKung Pow: Enter the Fist not worth its entrance feeLetters to the EditorMandy Moore finds her film niche in WalkMcNally transcends current pop scene with debut albumMen's Hoops: North Carolina (6-11, 2-5 ACC) at Georgia Tech (8-13, 1-7 ACC)News BriefsOUR VIEWS: Consensus OpinionParty worm hits campus computersProblems plague State StreetResearch ReviewRevamped leadership conference draws crowdSpikers coach Collier unexpectedly resignsSports BriefsStaff EditorialTech leads in environmental policiesTechs and the CityTech's first Relay for Life scheduled for April weekendThe Live List: Feb. 1 and beyond...Two BitsWhat's 'Eleemosynary'? It's DramaTech

A Three-Step Process To Facilitate the Annulation of Polycyclic Aromatic Hydrocarbons

A new efficient three-step process to annulate polycyclic aromatic hydrocarbons (PAHs) has been developed, providing access to PAHs with saturated rings that under current chemical methods would be difficult to produce in an efficient manner. This method relies on a palladium-catalyzed cross-coupling reaction of various brominated PAHs with cyclohexanone to yield α-arylated ketones, which are converted to regiospecific vinyl triflates and cyclized by a palladium-catalyzed intramolecular arene–vinyl triflate coupling to produce PAHs with incorporated saturated rings or “tetrahydroindeno-annulated” PAHs